Study on Influence Factors of ELISA for Chlorothalonil Residue

Abstract

Abstract: An indirect competitive ELISA method was established for determining chlorothalonil residues in vegetables through screening appropriate concentrations of antigen and antibody, exploring the effects of buffer system and pH on ELISA and evaluating the specificity and affinity of polyclonal antibodies. The results showed that the detection limit (IC20) of ELISA was 0.0123 ng/mL. The recovery rate of ELISA was 84.0%－89.8% with standard deviation of 3.0%－7.6%. The cross-reactivity (CR) with chlorothalonil analog was less than 0.01%.

Key words: chlorothalonil, ELISA, polyclonal antibody, cross-reactivity

CLC Number:

TS201.21

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Study on Influence Factors of ELISA for Chlorothalonil Residue

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