Abstract: Ultrasonic followed by microwave treatment was used to establish a novel method to extract total flavonoids from Radix Pueraria. The effects of five extractions, namely temperature during ultrasonic treatment, ultrasonic treatment time, microwave power and treatment time and solid-to-liquid ratio on the extraction yield of total flavonoids from Radix Pueraria were explored. The quantification of total flavonoids was conducted using a UV spectrophotometer. The results showed that the maximum yield of total flavonoids could reach 14.685 mg/g was attained by using 80% ethanol as the extraction solvent at a solid-to-liquid ratio of 1:25 under the assistance of 30 min ultrasonic at 50 ℃ followed by 9 min microwave treatment at 300 W power.

Key words: Radix Pueraria, flavonoids, ultrasonic, microwave