Folin-Ciocalteu colorimetric Determination of Total Polyphenol Content in Hovenia acerba Fruit

Abstract

Abstract: A quantitative Folin-Ciocalteu colorimetry method to determine total polyphenols in Hovenia acerba fruit was developed. The color reaction was carried out with 0.6 mL of Folin-Ciocalteu reagent and 1.5 mL of 20 g/100 mL aqueous Na2CO3 solution in darkness at 30 ℃ for 60 min. The detection wavelength was set as 750 nm. The linear range of the established standard curve was 0－8.47 mg/L. The relative standard deviations of stability, reproducibility, precision, and spike recovery rate of this method were all satisfactory. Thus, this method can be applicable to determine the total polyphenols of Hovenia acerba fruit.

Key words: Folin-Ciocalteu colorimetry, Hovenia acerba fruit, polyphenol

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Folin-Ciocalteu colorimetric Determination of Total Polyphenol Content in Hovenia acerba Fruit

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