Purification and Structural Identification of a Bioactive Polysaccharide Fraction from Ganoderma lucidum

Abstract

Abstract: A novel Ganoderma lucidum polysaccharide GLPS1a was purified sequentially by column chromatographies on DEAE-Sephadex A25 and Sepharose CL-6B gel. GLPS1a was eluted as a single symmetrical narrow peak on high-performance gel-permeation chromatography (HPGPC) and the average molgcular weight was 1.8×105 D. Das chromatographic analysis of absolute acid hydrolysate of GLPS1a suggested that its monosaccharide composition was composed of arabinose, galactose, glucose and xylose with a molar ratio of 4:2:10:1. Fourier-transform infrared (FT-IR), 1H and 13C NMR spectroscopy analyses revealed that GLPS1a had a backbone consisting ofβ-D-(1,3)-linked-D-glucosepyranosy1 residues with glycosy1 residues composed of β→(1,3) linked arabinoser,β-D-(1,4)-galcose,α-D-(1,2)-xylose and (1→6) linkedα-D-glucose residues.

Key words: Ganoderma lucidum polysaccharide, separation, purification, structural

CLC Number:

TS201.1

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