Isolation and Identification of a High Exopolysaccharide-producing Strain from Biological Soil Crusts on Sand Dunes

Abstract

Abstract: Twenty-seven stains producing exopolysaccharides were isolated from biological soil crusts in the Gurbantunggut Desert, Xinjiang by the traditional method. A strain with 7445.80 mg/L exopolysaccharide-producing activity was picked out of them by phenol-sulfuric acid method and named as XJ-27. Based on phenotypic and physiological-chemical characterization, XJ-27 was primarily identified as Bacillus thuringiensis and further confirmed through 16S rDNA sequence analysis, resulting in a 1452 bp PCR amplification sequence, homology comparison with NCBI databases using the software Blast and phylogenetic tree construction using the software MEGA 4.0.

Key words: biological soil crusts, exopolysaccharides, identification, Bacillus thuringiensis

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Isolation and Identification of a High Exopolysaccharide-producing Strain from Biological Soil Crusts on Sand Dunes

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