Separation of a Nucleoside Analogue from Rape Bee Pollen

Abstract

Abstract: In order to explore the nucleoside composition of rape bee pollen, the sample was subjected to extraction with 15% aqueous ethanol under the assistance of ultrasonic, TLC analysis using chloroform-methanol-ammonia (6.5:8.0:1.5, V/V) as the development solvent, chromatographic separation on D101 type macroporous adsorption resin column using 5% aqueous ethanol as the elution solvent, water saturated butanol extraction and high-performance liquid chromatographic (HPLC) purification and as a result, a single compound was obtained. The compound was identified as adenine by liquid chromatography-quadrupole-time of flight mass spectrometer (LC-Q-TOF) according to the data of accurate mass, double bond equivalent (DBE), spacing match and similarity score and by comparison of its retention time and UV spectrum with those of the reference substance.

Key words: rape bee pollen, nucleoside analogue, thin layer chromatography(TLC), separation, adenine

CLC Number:

References

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