Process Optimization for Cichoric Acid Extraction from Cichorium lntybus L. Shoots

Abstract

Abstract: The purpose of this study was to optimize the extraction of cichoric acid from Cichorium lntybus L. by methanol reflux extraction and then establish an HPLC method for determining cichoric acid content in Cichorium lntybus L. One-factor-at-a-time experiments were conducted to understand the effects of reflux temperature, methanol concentration, extraction time and solid-to-liquid ratio on extraction efficiency of cichoric acid. Based on this, an L9 (34) orthogonal array design was employed to optimize extraction efficiency of cichoric acid. The results demonstrated that the optimal conditions for cichoric acid extraction were reflux temperature of 60 ℃, solid-to-liquid ratio of 1:60 (g/mL), methanol concentration of 70%, and reflux duration of 1 h. Under the optimal conditions, the extraction efficiency of cichoric acid was 8.808 mg/g dry basis.

Key words: Cichorium lntybus L., cichoric acid, process optimization, HPLC

CLC Number:

中图分类号：S-3

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