Abstract: Staphylococcal enterotoxin (SE) is an important pathogenic factor for human food-borne diseases. Based on a couple of monoclonal antibodies with high sensitivity and affinity, a rapid, sensitive, specific DAS-ELISA method for the quantitative detection of SE was established. The linear regression equation was y＝4.074x－1.1888 (R2＝0.9892). The detection limit was 0.307 μg/mL. The average recovery rates for SE in skim milk powder were 103%－107% with relative standard deviation (RSD) of less than 10%. In addition, the method was also successfully used for monitoring the dynamic change of SE secretion in culture supernatants of Staphylococcus aureus isolates, indicating that it can be used not only for the detection of enterotoxin-producing strains but also for monitoring the secretion of SE. Moreover, the positive rates of the method for the detection of  SE in fresh milk and porcine lymphoid samples were 51.7% and 59.1%, respectively. The total coincidence rate between the results obtained by the method and imported kit was 92.5%. Therefore, the method was sensitive and efficient and could provide an effective technical support for monitoring food-borne pollution.

Key words: Staphylococcus aureus, staphylococcal enterotoxin, double antibody sandwich enzyme-linked immunosorbent assay(DAS-ELISA), detection method