Breeding of a High Melanin-producing Plectania Strain

Abstract

Abstract: In order to breed a Plectania strain with higher melanin-producing ability, the preparation and UV mutagenization of protoplasts from Plectania YM421 mycelium was optimized. Results indicated that maximum rate of protoplast formation was observed after 3 h hydrolysis of helicase added at a level of 2.5 g/100 mL at 33 ℃. After exposure to UV radiation for 15－25 s at the distance of 30 cm, mutant strain YM421-2# was obtained, which exhibited respective increases of melanin yield by 10.03% and biomass by 11.33% when compared with the start strain. This mutant strain can be used for industrial production of melanin as a candidate.

Key words: Plectania, protoplast, UV mutagenesis, melanin, biomass

References

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