Optimization of Hydrolysis Conditions for Preparation of Small Yellow Croaker-derived Angiotensin-I Converting Enzyme (ACE) Inhibitory Peptides Using Response Surface Methodology

Abstract

Abstract: In this study, small yellow croaker meat was hydrolyzed by trypsin (PTN 6.0S) to prepare angiotensin-I converting enzyme (ACE) inhibitory peptides with high activity. Using one-factor-at-a-time method and response surface analysis (RSA), the optimal hydrolysis conditions for maximum ACE inhibitory rate of hydrolysates were determined as follows: pH 7.0, 50 ℃, 16.5 h hydrolysis time, 5.6 g/100 mL substrate concentration, and 9‰ enzyme/substrate ratio. The ACE inhibitory rate of the hydrolysate obtained under these conditions was determined by RP-HPLC to be 87.36%.

Key words: small yellow croaker, trypsin, ACE inhibitory peptides, response surface methodology, RP-HPLC

CLC Number:

TS218

References

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