Abstract: Objective: To evaluate the antioxidant and analgesic activities of total flavonoids from the root bark of Morus alba. Methods: The antioxidant activity was evaluated by DPPH and hydroxyl free radical scavenging assays using VC as reference standard, and the analgesia activity by acetic acid-induced writhing response, formaldehyde-induced feet-licking response, and heat-induced pain threshold in mice. Results: The total flavonoids had obvious scavenging activity in vitro against DPPH and hydroxyl free radicals. These compounds resulted in a notable decrease in acetic acid-induced writhing number and feet-licking number induced by heat or formaldehyde and consequently had alleviating effect on pain in mice induced by chemicals and inflammatory reaction. Conclusion: Total flavonoids from the root bark of Morus alba have obvious antioxidant and analgesic activities.

Key words: Morus alba, flavonoids, analgesia activity, antioxidant activity