Determination of Dehydrogenase Activity in Four Moulds from Stored Cereal Grains

Abstract

Abstract: Based on the principle of TTC-dehydrogenase reductive reaction, a procedure for the determination of dehydrogenase activity was established in four typical moulds such as Aspergillus flavus sp., A. candidus sp., A. glaucus sp. and Penicillium sp. that are widely distributed in stored cereal grains. The results revealed that the dehydrogenase had stable reaction in Tris-HCl buffer with optimal TTC concentration of 0.2 g/100 mL and acetone volume of 80%. The optimal determination temperature for four moulds was 30 ℃ and the optimal pH for Aspergillus flavus sp. and A. candidus sp. was 11.0, while the optimal pH for A. glaucus sp. and Penicillium sp. was 10 and 10.5, respectively. The reaction duration for Aspergillus flavus sp. and Penicillium sp. was 1.5 h, whereas the reaction duration for A. candidus sp. and A. glaucus sp. was 2.5 h. A high correlation was observed between dehydrogenase activity and mould number during wheat mildew process.

Key words: grain mould, dehydrogenase, TTC, cell activity

CLC Number:

TS210

Yuan-Sen HU. Determination of Dehydrogenase Activity in Four Moulds from Stored Cereal Grains[J]. FOOD SCIENCE, 0, (): 207-211.

References

[1]梁文艳,王珂,阮清鸳,等.TTC-脱氢酶还原法测定铜绿微囊藻活性[J].环境科学学报.2008,28(9): 1745-1750.
[2]杨天佑,田静,黄新,等.TTC-脱氢酶测定法表征低温真空环境对细菌呼吸活性的影响[J].食品与机械.2008,24(6):32-35.
[3]朱南文,闵航,陈美慈,等. TTC-脱氢酶活性测定方法的探讨[J].中国沼气.1996,14(2):3-5.
[4]梁文艳,王珂,王金丽,等.TTC-脱氢酶还原法测定藻细胞活性的优化与评价[J].环境科学与管理.2009, 34(5):10-13.
[5]Cottrell H, Tetrazolium salt as a seed germination indicator. Nature.1947,159:748.
[6] Pearse A G E, Intracellular localization of dehydrogenase systems using monotetrazolium salts and metal chelation of their formazans[J]. Journal of Histochemistry and Cytochemistry.1957,5:515-527.
[7] Fred RB, Knight SG, The reduction of 2,3,5-triphenyltetrazolium chloride by Penicillihnnit chrysogenurn[J].Science.1949,109:169-170.
[8] Cycońa M, Segetb Z P, Kozdrójc J. Dehydrogenase activity as an indicator of different microbial responses to pesticide treated soils[J].Chemistry and Ecology.2010,26(4):243-250.
[9] Moreno B, Vivas A, Nogales R, et al. Restoring biochemical activity and bacterial diversity in a trichloroethylene contaminated soil: the reclamation effect of vermin composted olive wastes[J]. Environmental Science and Pollution Research International. 2009,16(3):253-264.
[10] Saide J A, Gilliland S E. Antioxidative activity of lactobacilli measured by oxygen radical absorbance capacity[J]. Journal of Dairy Science. 2005,88(4):1352-1357.
[11] 纪淑娟,浩淼,李东华.TTC法检测牛乳中抗生素快速检测试纸的制作[J].食品工业科技.2009,30 (3):322-324,327.
[12]刘伯宁,乔长晟,贾士儒.一种丝状真菌活细胞生物量测定方法的研究[J].药物生物技术.2006,13(1): 40-44.
[13]马宏瑞,赵敏,张景飞.假单胞菌DS-Ⅲ的脱氢酶活性与石油烃降解动力学特性[J].农业环境科学学报. 2007,26(2):559-562.
[14] Jeanette M. Norton, Mary K. Firestone metabolic status of bacteria and fungi in the rhizosphere of ponderosa pine seedlings[J].Applied and Environmental Microbiology.1991,57(4):1161-1167.

[1]	LIU Weiliang, MAO Ruixia, WANG Xuefeng, JI Yu, ZHAO Cunchao, WEI Guangqiang, HUANG Aixiang. Screening for Ethanol-Degrading Strains and Evaluation of Antialcoholic Efficacy of Fermented Milk Product with Them [J]. FOOD SCIENCE, 2020, 41(2): 107-113.
[2]	SHU Huizhen, TANG Zhiling, HAN Wei, CHEN Haiming, CHEN Weijun, HU Yueying, CHEN Wenxue. Antibacterial Activity and Mechanism of Caryophyllene against Brochothrix thermosphacta [J]. FOOD SCIENCE, 2020, 41(15): 31-38.
[3]	WANG Beibei, LI Haocong, SUN Wenjing, CUI Fengjie, WANG Daming,, QIAN Jingya, QI Xianghui. Isolation, Purification and Characterization of Membrane-Bound Gluconate Dehydrogenase from Serratia marcescens [J]. FOOD SCIENCE, 2019, 40(24): 101-109.
[4]	WANG Bo, LI Jixin, LI Jingwei, WANG Yu, HU Yanyun, JIANG Yumei. Effect of Postharvest Benzothiadiazole Treatment on Aroma Esters Derived from Amino Acid Metabolism and Metabolic Mechanism in ‘Yujinxiang’ Melon [J]. FOOD SCIENCE, 2018, 39(17): 212-220.
[5]	XIN Jianzeng, LI Zheng, LI Xin, LI Guixia, REN Chi, ZHANG Dequan. Effect of Glyceraldehyde-3-Phosphate on Color Stability and Metmyoglobin Reduction of Mutton [J]. FOOD SCIENCE, 2018, 39(16): 112-117.
[6]	ZHANG Shuang, ZHANG Nan, ZHU Liangqi, LI Chunbao. Energy Metabolism and Protein Phosphorylation of Pork, Beef and Chicken during the Early Postmortem Period [J]. FOOD SCIENCE, 2017, 38(9): 72-78.
[7]	LI Fen, SUN Daqing, ZHANG Liping,. Bioinformatic Analysis of Genes Encoding Lactate Dehydrogenase from Lactobacillus plantarum LY-78 [J]. FOOD SCIENCE, 2017, 38(8): 102-106.
[8]	ZHU Yibo, LU Rubin, CHENG Jun, WANG Ying, QI Bin, WANG Limei. Production of Optically Pure L-Phenyllactic Acid by Using Whole Cells of Recombinant Escherichia coli [J]. FOOD SCIENCE, 2017, 38(2): 59-64.
[9]	LAI Mulan, WAN Fang, ZHU Qiangyun, CHEN Xuelan. Effect and Molecular Mechanisms of Tween-80 on L-lysine Production in Corynebacterium crenatum [J]. FOOD SCIENCE, 2016, 37(23): 173-177.
[10]	CHENG Yayun, WANG Xin, ZHENG Lin, LI Guanhao, CUI Hushan, JIN Qing. Cloning and Expression of Mannitol Dehydrogenase Gene from Leuconostoc pseudomesenteroides [J]. FOOD SCIENCE, 2016, 37(13): 153-156.
[11]	NING Qingpeng, WANG Changqing, FANG Tian, BAI Yunyun, CHEN Tong. Preparation of Polypeptides with Sobering Effect from Peanut Meal by Enzymatic Hydrolysis [J]. FOOD SCIENCE, 2016, 37(13): 173-177.
[12]	ZHANG Min, JIANG Shaotong*, ZHENG Zhi, PAN Lijun, LI Xingjiang, LUO Shuizhong, WU Xuefeng. Cloning and Functional Characterization of Gene Promoters from Rhizopus oryzae AS 3.819 [J]. FOOD SCIENCE, 2015, 36(7): 71-78.
[13]	ZHAO Yunxia1, TAO Mingxuan1,*, CHENG Guangyu2, XING Jia1, LU Wenjuan1. Effects of Polysaccharides from Termitomyces albuminosus on Pathological Liver Ultrastructure and Gene Expression in Mice with Alcoholic Liver Injury [J]. FOOD SCIENCE, 2015, 36(5): 195-199.
[14]	LI Mingwei, SUN Jianxia, XU Wei, ZOU Feiyan, BAI Shun, ZHU Cuijuan, HU Yunfeng, JIAO Rui,WU Shi, OU Shiyi, FENG Mengge, BAI Weibin. Effect of Acrylamide on the Viability and Progesterone Biosynthesis Function of Rat R2C Leydig Cells [J]. FOOD SCIENCE, 2015, 36(17): 247-251.
[15]	FU Ting, WANG Dan, WAN Ji, TANG Yunming. Isolation, Purification and Partial Characterization of Alcohol Dehydrogenase from Pig Liver [J]. FOOD SCIENCE, 2015, 36(17): 179-184.