Multivariate Analysis of Pro- and Anti-Oxidant Properties of Raspberry and Blackberry from Different Varieties at Different Maturity Stages

Abstract

Abstract: The pro- and anti-oxidant properties of raspberry and blackberry from different varieties harvested at different stages of maturity were analyzed by multivariate analysis. The results of one-way analysis of variance (one-way ANOVA) showed that Triple Crown,, fruits contained even higher contents of GSH, total phenolics and total flavonoids than other varieties tested had lower total anti-oxidant capacity (TAC) at higher maturity. In addition, both variety and maturity showed more significant impact on the contents of GSH, total phenolics and MDA and hydroxyl radical-scavenging capacity in raspberry and blackberry. The results of principal component analysis (PCA) showed that variety had greater impact on TAC antioxidant capacity as compared with maturity. The results of correlation analysis and partial least squares regression (PLSR) indicated that H2O2 content was positively correlated with MDA content, GSH content or superoxide anion radical-scavenging capacity, whereas total phenolics content was positively correlated with TAC, hydroxyl radical-scavenging capacity or DPPH radical-scavenging capacity. The results of path coefficient analysis showed that the contents of total phenolics and total flavonoids had the most obvious effect on H2O2 content.

Key words: raspberry, blackberry, variety, maturity, anti-oxidant capacity, multivariate analysis

References

[1] 王文芝.树莓果实营养成分初报[J].西北园艺,2001,2:13-14.
[2] 向延菊,郑先哲,霍俊伟,等.树莓采后贮藏保鲜技术及其发展方向[J].农机化研究,2005, 01:220-221.
[3] 吴彩娥, 寇晓红, 王文生. 果实成熟衰老与保护酶系统一的关系[N]. 中国果菜, 2002, l6.
[4] YAMASAKI H , HESHIKI R , IKEHARA N et al . Leaf2goldening induced by high light in Ficus microcarpa L f, a tropical fig [J]. Plant Res , 1995 , 108 :171-180.
[5] 郭军战, 彭少兵, 陈铁山. 树莓和黑莓引种品种果实营养成分分析[J]. 西北林学院学报, 2004, 19 (1) :108-109.
[6] MICHELIS A D. Parameters affecting pre-cooling, freezing, storage and transport of red raspberry fruits, individually frozen in discontinuous tunnels. Comparison among five varieties of Rubus sp[J]. International Journal of Refrigeration, 2003, 26:586–592.
[7] HAFFNER K, ROSENFELD H J, SKREDE G, WANG L X. Quality of red raspberry Rubus idaeus L. cultivars after storage in controlled and normal atmospheres[J]. Postharvest Biology and Technology, 2002, 24:279-289.
[8] 王大伟, 向延菊. 成熟度对树莓贮藏保鲜的影响研究. Journal of Tarim University. 2005, 17(4): 36-38.
[9] WANG S Y, CHEN C T, WANG C Y. The influence of light and maturity on fruit quality and flavonoid content of red raspberries [J]. Food Chemistry, 2009, 112: 676-684.
[10]杨宏伟, 陈小燕, 张影, 等. 油桃采后衰老与病害发生中品质与活性氧代谢相关性分析. 食品科学,2011,32(20):244-248.
[11] 马殿君, 张黎斌. 红树莓的医疗保健作用及红树莓饮料的开发. 试验报告与理论研究, 2008, 11(2): 19-22.
[12] PANTELIDIS G E, VASILAKAKIS M, et al. Antioxidant capacity, phenol, anthocyanin and ascorbic acid contents in raspberries, blackberries, red currants, gooseberries and Cornelian cherries[J]. Food Chemistry, 2007, 102: 777-783.
[13] BRENNAN T, FRENKEL C. Involvement of hydrogen peroxide in the regulation of senescence in pear [J]. Plant Physiology, 1977, 59: 411-416.
[14] WANG Y S., TIAN S P , XU Y , et al. Changes in the activities of pro- andanti-oxidant enzymes in peach fruit inoculated with Cryptococcus laurentii or Penicillium expansum at 0 or 20 °C [J]. Postharvest Biology and Technology, 2004, 34: 21-28.
[15] BENZIE I F F , STRAIN J J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay [J]. Analytical Biochemistry, 1996, 239:70-76.
[16] PETER R R, KREPSKY P B, SIQUEIRA J M , et al. Nitric oxide and cyclooxygenase may participate in the analgesic and anti-inflammatory effect of the cucurbitacins fraction from Wilbrandia ebracteata [J]. Life Sciences, 2003, 73: 2185-2197.
[17] BLOIS M S. Antioxidant determination by the use of a stable free radical [J]. Nature, 1958, 181:1199-1200.
[18] 李莉, 王友升, 张帆, 等. 采前钙处理对树莓果实贮藏效果及清除自由基能力的影响[J]. 吉林农业大学学报, 2009, 31(5): 616-620, 627.
[19] HUANG R H, XIA R X, HU L M, et al. Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation [J]. Scientia Horticulturae, 2007, 113: 166-172.
[20] SINGLETON V L, ROSSI J A. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents [J]. American Journal of Enology and Viticulture, 1965, 16, 144–158.
[21] 朱昱燕, 王友升, 赵茜, 等. 槐花中抗氧化及清除自由基活性物质的提取条件研究[J]. 食品工业科技, 2009, 30(12): 130-132, 135.
[22] PIERCE K M, HOPE J L, et al. A principal component analysis based method to discover chemical differences in comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC×GC-TOFMS) separations of metabolites in plant samples [J]. Talanta, 2006, 70: 797-804.
[23] ASADA K, TAKAHASHI M. Production and scavenging of active oxygen in photosynthesis , Elsevier Science Publishers ,Amsterdam ,1987 : 227-28.