Collagen Content of Velvet Antlers at Different Growth Stages and Optimization of Extraction Process

Abstract

Abstract: Velvet antlers were selected as raw materials to extract collagen using enzyme pepsin. Collagen contents at different growth stages were also compared. Results showed that content and the yield of collagen in two branched antler were 65.8 mg/g and 4.53%, respectively, which were significantly higher than three branched antler (P＜0.05). Factors of extraction time, enzyme addition and liquid-solid ratio were chosen to research the effects on yield of collagen and Box-Behnken experiment was designed to optimize the extraction conditions. The results showed that extraction time for 52 h, enzyme addition with 5%, liquid-solid ratio at 23:1 (mL/g) were the optimal conditions, the yield of collagen in two-branched antler was 5.92%.

Key words: antler, collagen, extraction, optimization

CLC Number:

TS201.7

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Collagen Content of Velvet Antlers at Different Growth Stages and Optimization of Extraction Process

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