Isolation and Identification of a Thermophilic Bacterium from Hot Spring in Changbai Mountain

doi:10.7506/spkx1002-6630-201321055

Abstract

Abstract:

In this work, physiological and biochemical properties of a thermophilic bacterium named T1 isolated from hot
springs in Changbai Mountain were analyzed. The results showed that the cells were Gram-positive, and non-motile rods.
The optimal growth temperature was 65 ℃ and pH 6.5. The strain used glucose, maltose as carbon and energy sources. The
16S rRNA sequence analysis showed that the sequence shared 99% similarity with that of Geobacillus stearothermophilus.
The G + C content of T1 was 7.5%, which was less than that of Geobacillus stearothermophilus. Preliminary identification
of the strain was a member of Geobacillus stearothermophilus.

Key words: thermophilic bacterium, 16S rRNA, isolation and identification

CLC Number:

LIU Jing-sheng，XU Zhi-chao，LIU Hui-min，CAI Dan，ZHENG Ming-zhu. Isolation and Identification of a Thermophilic Bacterium from Hot Spring in Changbai Mountain[J]. FOOD SCIENCE, 2013, 34(21): 274-277.

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