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Effect of Rhizoma Gastrodiae on Key Enenzyme Activities Involved in the Biosynthesis of Exopolysaccharides from Grifola frondosa in Submerged Culture

HE Zong-yi,WU Tian-xiang,XU Xiao-bao   

  1. 1. Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;2. School of Life Sciences, Guizhou University,Guiyang 550025, China;3. School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China
  • Online:2013-06-15 Published:2013-06-03


The effect of Rhizoma Gastrodiae on the biosynthesis of expolysaccharide (EPS) from Grifola frondosa in
submerged culture was studied. Total polysaccharide content was determined by phenol-sulphuric acid method. The results
showed that Rhizoma Gastrodiae at 7 g/L significantly promoted the biosynthesis of EPS in Grifola frondosa when compared
with blank control, increasing EPS yield from 3.72 g/L to 3.91 g/L. The measured activities of two key enzymes involved in
the synthesis of EPS indicated that after fermentation in the presence of Rhizoma Gastrodiae, PGI activity was decreased,
although α-PGM activity showed no obvious change.

Key words: Grifola frondosa;Rhizoma Gastrodiae;exopolysaccharide;PGI;&alpha, -PGM