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Development and Application of Colloidal Gold Rapid Test Strip for Chloramphenicol in Aquatic Products

SANG Li-ya,WU Mao-sheng,HUANG Yi-wen,SHENG Hui-ping,LIU Ai-chun   

  1. 1. Hangzhou Nankai Biotech Co. Ltd., Hangzhou 310022, China;2. Fujian Provincial Marine Environmental and Fisheries Resources Monitoring Center, Fuzhou 350003, China;3. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China
  • Online:2013-06-25 Published:2013-06-17

Abstract:

A colloidal gold test strip for rapid detection of chloramphenicol in aquatic products has been developed and
applied. Colloidal gold particles were prepared and labeled to an anti-chloramphenicol (CAP) monoclonal (MAb) antibody.
Optimum concentrations of goat anti-mouse IgG, chloramphenicol-bovine serum albumin (CAP-BSA) and the gold labeled
antibody were established by repeated testing before coating a nitrocellulose (NC) membrane and a colloidal gold conjugate
pad. After being dried at 37 ℃ for 32 h or 25 ℃ for 7 d, the NC membrane, together with a sample pad, a colloidal gold
conjugate pad, a absorbent pad and other accessories, was used to fabricate a colloidal gold test strip. The detection limit of
the test strip for chloramphenicol was 0.3 μg/kg, and false positive rate (FPR) and false negative rate (FNR) were 4% and 2%
respectively. Its period of validity was more than 6 months at room temperature.

Key words: chloramphenicol, colloidal gold, rapid test, aquatic products