FOOD SCIENCE ›› 2016, Vol. 37 ›› Issue (20): 167-171.doi: 10.7506/spkx1002-6630-201620028

• Safety Detection • Previous Articles     Next Articles

Determination of Perchlorate in Chicken Meat and Liver by Ion Exchange High Performance Liquid Chromatography-Tandem Mass Spectrometry

SHAO Linzhi, WU Yingxuan, OUYANG Shaolun, LIN Feng   

  1. 1. Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau,
    Guangzhou 510623, China; 2. Key Laboratory of Animals and Plants and Food Import and Export of Technical Measures in
    Guangdong Province, Guangzhou 510623, China
  • Received:2016-10-27 Revised:2016-10-27 Online:2016-10-25 Published:2016-12-01


A method was developed for the determination of perchlorate in chicken meat and liver by ion- exchange high
performance liquid chromatography-tandem mass spectrometry (IC-HPLC-MS/MS). After adding isotopically labeled
internal standards to it, the sample was extracted with 1% acetic acid-acetonitrile, and then purified by solid-phase extraction
column. The target compound was separated on an IC-PakTM Anion HR column (4.6 mm × 75 mm, 6 μm) using a mobile
phase consisting of acetonitrile and 100 mmol/L aqueous ammonium acetate, and detected by tandem mass spectrometry
in negative electrospray ionization and multiple reaction monitoring (MRM) mode. The quantification of perchlorate was
performed by the internal standard method. The calibration curves showed good linearity in the concentration range of
0.2–10 μg/L with a limit of quantification (LOQ) of 3.0 μg/kg. The recoveries in chicken meat and liver at three spiked levels
were in the range of 91.8%–96.0% with relative standard deviations (RSDs) between 1.8% and 6.5%. With the advantages
of convenience, high sensitivity, strong anti-interference capability and good recovery and repeatability, the method was
suitable for the analysis of perchlorate in chicken meat and liver.

Key words: perchlorate, isotope dilution technique, solid-phase extraction, high performance liquid chromatography-tandem mass spectrometry, chicken meat and liver

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