Abstract: Abstract: A method for the simultaneous determination of 10 preservatives, including peimisine, carbendazim, thiophanate-methyl, thiabendazole, iprodione, imazalil, benomyl, procymidone, chlorothalonil and pyrimethanil in fruits was developed by using ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were extracted by QuEChERS method. The UPLC-MS/MS method was performed with ACQUITY UPLC BEH C18 column using acetonitrile solution (contain 0.1% formic acid) as mobile phase by gradient elution. The target compounds were determined by UPLC-MS/MS under multiple reaction monitoring (MRM) mode, and then quantified by external standard method using the standard solution．The calibration curves showed good linearity with correlation coefficients greater than 0.992. The recoveries of the 10 preservatives spiked at three levels ranged from 77.8% to 105%, with the relative standard deviations (RSDs) less than 8.9%. The limits of detection (LODs，S/N≥3) were 0.02 – 1.5 μg/kg and the limits of quantity (LOQs，S/N≥10) were 0.1 – 4.1 μg/kg for 10 preservatives in fruits. The established method was simple, sensitive, accurate and reliable, and could applied to the simultaneous determination of multi preservatives residues analysis in fruits．

Key words: preservatives, QuEChERS, fruits, liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)