Conversion of Linoleic Acid to Conjugated Linoleic Acid by Lactobacillus plantarum p-8

Abstract

Abstract: cis9, trans11-Conjugated linoleic acid (c9, t11-CLA), trans10, cis12-Conjugated linoleic acid (t10, c12-CLA) and trans9, trans11-Conjugated linoleic acid (t9, t11-CLA) are three isomers that play probiotic functions, its can produced by bioconversion LA. In this study, the ability and mechanism of conversion LA to CLA by cells, disrupted cells solution and the linoleate isomerase family of Lactobacillus plantarum (L. plantarum) p-8. The result shown that L. plantarum p-8 could low-efficiently produce c9, t11-CLA, t10, c12-CLA and t9, t11-CLA when the MRS medium containing LA and the disrupted cells solution catalyzed LA,and only a small amount of t10, c12-CLA was found in the cells. The result of RT-qPCR shown that, lower expression levels of linoleate isomerase family may be responsible for lower CLA production. Only members of the independently expressed recombinant linoleate isomerase family, Flavin denine dinucleotide (FAD) and Nicotinamide adenine dinucleotide (NAD+) are present to complete conversion LA to c9, t11-CLA, t10, c12-CLA and t9, t11-CLA, and it conversion mechanism includes hydration, dehydrogenation, double bond shift, oxidation and dehydration. Homologous modeling of linoleic acid hydrase has three domains. The substrate binding site and FAD site are located in the hydrophobic cavity at the junction of the three domains. M76 and Y180 are two essential residues.

Key words: cis9, trans11-Conjugated linoleic acid (c9, t11-CLA), trans10, cis12-Conjugated linoleic acid (t10, c12-CLA), trans9, trans11-Conjugated linoleic acid (t9, t11-CLA), Fatty acid hydratase, Prokaryotic expression, Homologous modeling

CLC Number:

Wei Zhao Inner MongoliaAgricultural heping zhang Inner MongoliaAgricultural. Conversion of Linoleic Acid to Conjugated Linoleic Acid by Lactobacillus plantarum p-8[J]. FOOD SCIENCE, 0, (): 0-0.

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