藏灵菇嗜热链球菌胞外多糖对人结肠癌细胞的增殖抑制

食品科学 ›› 2008, Vol. 29 ›› Issue (4): 405-408.

藏灵菇嗜热链球菌胞外多糖对人结肠癌细胞的增殖抑制

马杉姗, 王世平, 连正兴, 韩红兵, 刘慧

中国农业大学食品科学与营养工程学院；中国农业大学动物科技学院；北京农学院食品科学系；中国农业大学农业生物技术国家重点实验室；

出版日期:2008-04-15 发布日期:2011-08-24

Study on Growth Inhibiting Effect of of Streptococcus thermophilus Exopolysaccharide from Tibetan Kefir on Human Colon Cancer Cells

MA 杉Shan, WANG Shi-Ping, LIAN Zheng-Xing, HAN Hong-Bing, LIU Hui

1.College of Food Science and Nutritional Engineering, China Agricultural University; 2.College of Animal Science and Technology, China Agricultural University; 3.State Key Laboratories for Agro-biotechnology, China Agricultural University; 4.Department of Food Science, Beijing Agricultural College

Online:2008-04-15 Published:2011-08-24

摘要/Abstract

摘要： 探讨从藏灵菇中筛选的嗜热链球菌(Streptococcusthermophilus)所产胞外多糖(exopolysaccharide,EPS)对人结肠癌HCT-8细胞增殖的影响。采用CCK-8法测定EPS对HCT-8细胞生长的抑制率;形态学观察EPS对HCT-8细胞的杀伤作用;流式细胞术检测EPS对HCT-8细胞周期的影响。结果显示:EPS对HCT-8细胞增殖有明显的抑制作用,且呈时间-剂量依赖性;倒置显微镜下观察部分细胞脱壁、核碎裂、固缩,细胞悬浮坏死;细胞周期分析显示G1期细胞百分率增大。说明EPS通过直接杀伤人结肠癌HCT-8细胞,使细胞周期阻滞于G1期而抑制细胞增殖,从而发挥抗肿瘤作用。

关键词: 嗜热链球菌, 胞外多糖, 人结肠癌细胞, 增殖抑制, 细胞周期

Abstract: To investigate the proliferation effect of exopolysaccharide(EPS)produced from Streptococcus thermophilus of Tibetan kefir on human colon cancer HCT-8 cells. CCK-8 assay was used to examine the effect of EPS in anti-proliferation, killing effect of EPS was evaluated through morphology identification combined with fluorescence microscopy observation, the cell cycle pattern was studied by flow cytometry. CCK-8 assay showed that the proliferation of HCT-8 cells was inhibited apparently after treatment with EPS, and in a time- and dose-dependent manner. EPS treatment resulted in morphological changes, including cell shrinkage, membrane blebbing, nuclear condensation, boundary aggregation or split, the necrotic cells increased along with the elevation of EPS concentration, and leading to cell cycle arrest at G1 phase. EPS showed that its anti- tumer effect by way of direct killing effects and make cells arresting the G1 phase to inhibiting the cell growth.

Key words: Streptococcus thermophilus, exopolysaccharide, human colon cancer cells, anti-proliferation, cell cycle

马杉姗, 王世平, 连正兴, 韩红兵, 刘慧. 藏灵菇嗜热链球菌胞外多糖对人结肠癌细胞的增殖抑制[J]. 食品科学, 2008, 29(4): 405-408.

MA 杉Shan, WANG Shi-Ping, LIAN Zheng-Xing, HAN Hong-Bing, LIU Hui. Study on Growth Inhibiting Effect of of Streptococcus thermophilus Exopolysaccharide from Tibetan Kefir on Human Colon Cancer Cells[J]. FOOD SCIENCE, 2008, 29(4): 405-408.

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