不同破壁方法对提取废啤酒酵母RNA的影响

食品科学 ›› 2008, Vol. 29 ›› Issue (3): 204-206.

不同破壁方法对提取废啤酒酵母RNA的影响

李艳, 阮南, 杨翠竹

河北科技大学生物科学与工程学院；河北科技大学生物科学与工程学院河北石家庄050018；河北石家庄050018；

出版日期:2008-03-15 发布日期:2011-08-24

Effects of Differrent Methods of Breaking Cells Wall on Yeast RNA Extraction

LI Yan, RUAN 南, YANG Cui-Zhu

College of Biology Sience and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China

Online:2008-03-15 Published:2011-08-24

摘要/Abstract

摘要： 从啤酒酵母中提取核酸的方法有很多,常用的是盐法和碱法。本研究对这两种方法提取核酸效果进行了比较,实验说明结果碱法对酵母破壁效果好于盐法,但是核酸提取的综合效果却不如盐法。

关键词: 酵母破壁, 碱破壁, 盐破壁, 核酸提取

Abstract: There are many methods for extracting RNA from brewing yeast. The salt and the alkali are often used. The two methods for extracting RNA from brewing yeast were compared there. Through the experiment it was found that alkail is better to break yeast, but worse to extract RNA.

Key words: broken yeast, alkali breaking cell wall, salt breaking cell wall, RNA extraction

李艳, 阮南, 杨翠竹. 不同破壁方法对提取废啤酒酵母RNA的影响[J]. 食品科学, 2008, 29(3): 204-206.

LI Yan, RUAN 南, YANG Cui-Zhu. Effects of Differrent Methods of Breaking Cells Wall on Yeast RNA Extraction[J]. FOOD SCIENCE, 2008, 29(3): 204-206.