关键词: 丁香, 灰葡萄孢, 提取方法, 超声波

Abstract: The natural plant extracts have inhibitory effects against pathogenic bacteria of post-harvest fruits and vegetables.It is validated that Flos Caryophlli extract can significantly inhibit the main low-temperature pathogenic bacterium Botrytis gray of post-harvest grape.Extraction of antibacterial ingredients from Flos Caryophlli by ultrasonic was studied.Using single factor and orthogonal design,the effects of solvent selection,solvent concentration,material to solvent ratio,ultrasonic temperature,ultrasonic power,and time on Flos Caryophlli extracts with growth rate method determining antibacterial rate.The results showed that on the conditions of 80% ethanol as extraction solvent,material to ethanol ratio 1:15,temperature 55 ℃,ultrasonic power 210 W,and time 60 min,the antibacterial activity of extracts reached the highest.

Key words: Flos Caryophlli, Botrytis cinerea, extraction method, ultrasonic