食品科学 ›› 2004, Vol. 25 ›› Issue (8): 66-69.

• 基础研究 • 上一篇    下一篇

野生松乳菇菌种分离与培养特性的研究

 周传云, 廖兴华, 谭周进, 彭晔峰   

  1. 湖南农业大学食品科技学院
  • 出版日期:2004-08-15 发布日期:2011-10-24

Study on the Strain Breeding and Cultivating Conditions of Wild Lactarius deliciosus

 ZHOU  Chuan-Yun, LIAO  Xing-Hua, TAN  Zhou-Jin, PENG  Ye-Feng   

  1. College of Food Science and Technology, Hunan Agriculture University
  • Online:2004-08-15 Published:2011-10-24

摘要: 以新鲜野生松乳菇子实体为材料,分别取其组织和孢子进行菌种分离,获得纯菌株。经固体培养和液体培养研究其人工培养特性,得知:以葡萄糖和蔗糖为碳源,蛋白胨或硝酸钾为氮源,pH7.5~8,26℃等条件下菌丝生长很旺盛,但不形成子实体。当以松树锯木屑或湿松树根或锯木屑加糖为培养料,并在恒温(25℃)、恒湿(95%)、光照培养条件下,不仅菌丝生长旺盛,而且能长出大拇指大小的菌蕾。

关键词: 松乳菇, 菌种分离, 食药用菌, 人工栽培

Abstract: Strain Lactarius deliciosus was obtained from tissue and spore of wild Lactarius deliciosus. The study on cultivatingconditions was conducted. The results showed that glucose and sucrose were the optimal carbon sources, peptone and KNO3 werethe optimal nitrogen sources. The optimal pH value was 7.5 to 8.0 and the optimial temperature 26℃ for mycelium growth ofLactarius deliciosus. When cultured with pine tree timber sawdust or wet pine root or wood sawdust at 25℃ constant temperature,95% constant humidity and illuminatied cultivating conditions, mycelium of Lactarius deliciosus grew well and mushroom alsocould be polarized.

Key words: Lactarius deliciosus, strain breeding, edible and medical fungus, artifical culture