食品科学 ›› 2004, Vol. 25 ›› Issue (9): 144-146.

• 分析检测 • 上一篇    下一篇

出血性大肠杆菌H7鞭毛抗原的诱导恢复及鉴定

 王志强, 许龙岩, 李志勇, 凌莉   

  1. 广州出入境检验检疫局
  • 出版日期:2004-09-15 发布日期:2011-10-24

Induction Variation of H7 Flagellum Antigen and Identification of EHEC O157:H7 from Imported Frozen Chicken Wing

 WANG  Zhi-Qiang, XU  Long-Yan, LI  Zhi-Yong, LING  Li   

  1. Guangzhou Entry-exit Inspection and Quarantine Bureau
  • Online:2004-09-15 Published:2011-10-24

摘要: 从进口冻鸡翅中分离获得一株发酵山梨醇、缺失H7鞭毛抗原的出血性大肠杆菌O157:H7变异株,采用0.4%强营养软琼脂对该菌进行连续5代诱导培养,使其H7鞭毛抗原成功恢复。综合该菌的生物学特性、血清学及毒理实验结果确证为大肠杆菌O157:H7。该菌的检出为大肠杆菌O157:H7变异株的鉴定提供了有益参考。

关键词: 出血性大肠杆菌, 鞭毛抗原诱导, 鉴定

Abstract: A variation EHEC O157:H7 strain was isolated from imported frozen chicken wing, while fermented sorbitol and lackedH7 flagellum antigen, was identified as EHEC O157:H7 by the combination of biological characters, serological result and toxicologicaltest. And the lost H7 flagellum antigen of EHEC O157:H7 was successfully induced after continuous 5-generation induction via0.4% high-nutrition soft agar. Our study was a useful reference for the identification of other variation EHEC O157:H7 strain.

Key words: EHEC O157:H7, induction of flagellum antigen, identification