食品科学 ›› 2004, Vol. 25 ›› Issue (10): 280-283.

• 分析检测 • 上一篇    下一篇

乌桕叶主要黄酮类物质的定性定量

 霍光华, 高荫榆   

  1. 江西农业大学生物工程系,南昌大学生命科学学院
  • 出版日期:2004-10-15 发布日期:2011-10-24

The Qualitative and Quantitative Analysis of Main Flavonoids from Chinese Tallow Leaf

 HUO  Guang-Hua, GAO  Yin-Yu   

  1. 1.Bioengineering Department, Jiangxi Agricultural University;2.Life Science College, Nanchang University
  • Online:2004-10-15 Published:2011-10-24

摘要: 通过硅胶和Sephadex LH-20凝胶柱色谱分离乌桕叶黄酮类物质,HPLC鉴别其纯度和定量,UV、IR、NMR、LC-MS定性,结果显示乌桕叶所含主要黄酮类物质为槲皮素、山柰酚及其组成的甙,其中槲皮素、山柰酚和槲皮素甙百分含量分别为0.0965、0.1232、0.6975。

关键词: 乌桕叶, 黄酮类物质, 定性定量

Abstract: Flavonoids were separated from Chinese tallow leaf (CTL) using silica and sephadex LH-20 gel columnchromatography, identified their purity and analyzed quantitatively using HPLC, analyzed quanlitatively using UV,IR,NMR,LC-MS. The results showed that CTL flavonoids consist of quercetin, kaempferol and their glycosides. Contents (g/100g drymass) of quercetin, kaempferol and quercetin glycoside are 0.0965, 0.1232,0.6975 in CTL respectively.

Key words: Chinese tallow leaf, Flavonoids, Qualitative and quantitative analysis