双缩脲法测定荞麦蛋白质

食品科学 ›› 2004, Vol. 25 ›› Issue (10): 258-261.

双缩脲法测定荞麦蛋白质

刘邻渭, 陶健, 毕磊

西北农林科技大学食品科学与工程学院

出版日期:2004-10-15 发布日期:2011-10-24

Quantitative Analysis of Buckwheat Protein with Biuret Method

LIU Lin-Wei, TAO Jian, BI Lei

Food Science and Technology Institute,Northwest Sci-Tech University of Agriculture and Forestry

Online:2004-10-15 Published:2011-10-24

摘要/Abstract

摘要： 本文介绍了一种双缩脲试剂和分析方法。探讨了如何正确使用它测定荞麦蛋白质含量。结果表明:①这种试剂和方法适用面宽、精密度高、回收率>95%,采用牛血清蛋白为标准物质制作标准曲线时,分析结果中的蛋白质为碱液可溶性蛋白质,采用代表性很强的样品(已知其总蛋白质含量)为标准物质制作标准曲线时,分析结果中的蛋白质为总蛋白质。②白花荞麦仁中总蛋白质含量为11.6%,脱除麦仁表层所留下的麦仁内部的蛋白质含量为5.6%,荞麦仁的碱液可溶蛋白的含量为7.6%,脱除麦仁表层所留下的麦仁内部的减液可溶的蛋白质含量为2.5%。

关键词: 双缩脲, 荞麦蛋白质, 白花荞麦

Abstract: A new Biuret reagent and test procedure was introdued in this paper. How proper using of the method to assay proteincontained in Buckwheat was investigated also. The results had showed two facts ① The reagent and the procedure werepracticable for analysis of different samples, with high precision (Cv<0.5%) and fair reclamation (>%). As BSA being thestandard, the analysis result is alkaline soluble protein content of the sample. As the correct representative sample being thestandard, the analysis result is total protein content of the sample. ② The protein content of White Flower Buckwheat kernelwas 11.6%. The protein content of the inner part of the kernel was 5.6%. The alkaline soluble protein content of White FlowerBuckwheat kernel was 7.6%. The alkaline soluble protein content of the inner part of the kernel was 2.5%.

Key words: biuret, buckwheat protein, flower buckwheat

刘邻渭, 陶健, 毕磊. 双缩脲法测定荞麦蛋白质[J]. 食品科学, 2004, 25(10): 258-261.

LIU Lin-Wei, TAO Jian, BI Lei. Quantitative Analysis of Buckwheat Protein with Biuret Method[J]. FOOD SCIENCE, 2004, 25(10): 258-261.

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