食品科学 ›› 2003, Vol. 24 ›› Issue (9): 103-106.

• 工艺技术 • 上一篇    下一篇

乳酸乳球菌发酵液中乳链菌肽的分离纯化

 吴琼, 生英涛, 唐名山, 谷体华, 葛庆燕   

  1. 山东轻工业学院食品与生物工程学院
  • 出版日期:2003-09-15 发布日期:2011-12-13

Study on Isolation and Purification of Nisin Adsorbed to Lactococcus lactis subsp Lactis SQ80

 WU  Qiong, SHENG  Ying-Tao, TANG  Ming-Shan, GU  Ti-Hua, GE  Qing-Yan   

  • Online:2003-09-15 Published:2011-12-13

摘要: 对乳酸乳球菌乳酸亚种SQ80随pH的改变吸附乳链菌肽的规律进行了研究,pH6.5时吸附率达91.5%,pH3以下吸附率为0。通过调整pH,使乳酸乳球菌选择性地吸附、释放乳链菌肽,达到了较好的分离纯化效果,HPLC检测显示单一尖峰,乳链菌肽回收率58.2%,纯化倍数75。

关键词: 乳酸乳球菌乳酸亚种, 乳链菌肽, 分离纯化, 吸附率

Abstract: The adsorption rate of nisin to Lactococcus lactis subsp. Lactis SQ80 at different pH conditions was studied. AtpH 6.5, it was 91.5%, the biggest one, but at pH<3.0, nisin wasn’t adsorbed at all. By changing pH , SQ80 cells could adsorbor release nisin selectively. The efficiency of isolation and purification was high. HPLC showed the single peak.The spelifil activityof nisin was increased 75 fold with a yield of 58.2%.

Key words: Lactococcus lactis subsp. Lactis, nisin, isolation and purification, adsorption rate