改良热酚水法制备大肠杆菌O157:H7脂多糖抗原的研究

食品科学 ›› 2006, Vol. 27 ›› Issue (10): 273-275.

改良热酚水法制备大肠杆菌O157:H7脂多糖抗原的研究

宋宏新, 刘晓阳, 李宏

陕西科技大学生命科学与工程学院

出版日期:2006-10-15 发布日期:2011-11-16

Preparation of Specific Lipopolysaccharide Antigen of Enterohemorrhagic Escherichia coli O157:H7 with Advanced Hot Phenol-water Method

SONG Hong-Xin, Liu-Xiao-Yang, Li-Hong

College of Life Science and Engineering, Shaanxi University of Science and Technology, Xianyang 712081, China

Online:2006-10-15 Published:2011-11-16

摘要/Abstract

摘要： 采用热酚水法提取肠出血性大肠杆菌(EHEC)O157:H7的脂多糖(LPS),经浓缩酶解,离心分离得纯化LPS,酸解制得O-特异性侧链(O-PS),能有效去除核酸及杂蛋白,抗原具有鲎试剂凝集活性及对产蛋鸡有较强的免疫原性,建立了一种改良热酚水法提取制备O157:H7特异性脂多糖抗原的方法。

关键词: EHEC O157:H7, 热酚水法, 脂多糖抗原制备

Abstract: The LPS antigen was prepared from EHEC O157:H7 strain 933 with hot phenol water method and purified by enzyme hydrolyzing with DNase and RNase, and then hydrolyzed by 1.5% acetic acid for getting OPS. The O157:H7 LPS antigen has high purity specificity, has activity of agglutinating limulus amebocyte lysate and high immunoreactivity for chicken. They could be used to produce specific anti-LPS antibody.

Key words: , EHEC O157:H7； hot phenol-water method； LPS antigen preparation；

宋宏新, 刘晓阳, 李宏. 改良热酚水法制备大肠杆菌O157:H7脂多糖抗原的研究[J]. 食品科学, 2006, 27(10): 273-275.

SONG Hong-Xin, Liu-Xiao-Yang, Li-Hong. Preparation of Specific Lipopolysaccharide Antigen of Enterohemorrhagic Escherichia coli O157:H7 with Advanced Hot Phenol-water Method[J]. FOOD SCIENCE, 2006, 27(10): 273-275.