双表达解毒酶基因工程菌的酶活性分析及其应用

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 33-37.

• 基础研究 • 下一篇

双表达解毒酶基因工程菌的酶活性分析及其应用

郑玉芝，郭成君，张建亮，郭新民，乔传令

中国科学院动物研究所农业虫鼠害综合治理国家重点实验室；中国人民大学环境学院；

出版日期:2006-12-15 发布日期:2011-11-23

Application and Activity Assay of Co-expressed Organophosphorus Hydrolase and Carboxylesterase(B1) Produced by Genetic Engineering Bacteria

ZHENG Yu-zhi1,GUO Cheng-jun2,ZHANG Jian-liang1, GUO Xin-min2,QIAO Chuan-ling1

1.Institute of Zoology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Chinese Academy of Science, Graduate University of Chinese Academy of Sciences, Beijing 100080, China;2. School of Environment, Renmin University of China, Beijing 100872, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 羧酸酯酶和有机磷水解酶可以水解不同的底物,为了获得降解谱广的解毒酶系,将两种酶共表达在同一载体。采用正交法对有机磷水解酶酶活性的分析条件进行了优化,以对硫磷为底物,底物浓度2×10-4mol/L、反应温度25℃、反应时间10min、pH8.0是测定酶活性最佳条件。在pH7.0～8.0的体系中存放一年活力可以保持50%以上。用于蚊子解毒实验,95%以上的对硫磷中毒蚊幼虫可以救活。在生物传感器等其它领域也都有应用。

关键词: 羧酸酯酶, 有机磷水解酶, 酶活性测定

Abstract: Carboxylesterase and organophosphorus hydrolase (OPH) are capable of hydrolyzing and detoxifying different pesticides. In order to get the widest substrate spectrum organophosphorus hydrolase and carboxylesterase (B1) were co-expressed in one genetic engineering bacteria. The activity of OPH was assayed by orthogonal test, and the optimum condition were substrate concentration 2×10-4mol/L, temperature 25℃, reaction time 10min and pH8.0. In the detoxification of parathion on mosquitoes, 95% of the mosquitoes can be survived. And it has been used in biosensor for pesticide detection.

Key words: carboxylesterase, organophosphorus hydrolase, enzyme activity assay

郑玉芝，郭成君，张建亮，郭新民，乔传令. 双表达解毒酶基因工程菌的酶活性分析及其应用[J]. 食品科学, 2006, 27(12): 33-37.

ZHENG Yu-zhi1,GUO Cheng-jun2,ZHANG Jian-liang1, GUO Xin-min2,QIAO Chuan-ling1. Application and Activity Assay of Co-expressed Organophosphorus Hydrolase and Carboxylesterase(B1) Produced by Genetic Engineering Bacteria[J]. FOOD SCIENCE, 2006, 27(12): 33-37.

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双表达解毒酶基因工程菌的酶活性分析及其应用

Application and Activity Assay of Co-expressed Organophosphorus Hydrolase and Carboxylesterase(B1) Produced by Genetic Engineering Bacteria

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