食品科学 ›› 2006, Vol. 27 ›› Issue (11): 248-253.

• 工艺技术 • 上一篇    下一篇

缓释葡萄糖氧化酶最适制备条件初探

 王学东, 辛锋,  夏文水,  沈炯, 郇美丽,  张莉萍   

  1. 武汉工业学院食品科技学院
  • 出版日期:2006-11-15 发布日期:2011-11-28

Pilot Study on the Optimum Conditions to Prepare Controlled-release Glucose Oxidase

 WANG  Xue-Dong, XIN  Feng,   Xia-Wen-Shui,   Shen-Jiong, HUAN  Mei-Li,   Zhang-Li-Ping   

  1. College of Food Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China
  • Online:2006-11-15 Published:2011-11-28

摘要: 以壳聚糖和海藻酸钠为复合缓释壁材,对葡萄糖氧化酶(GOD)进行微胶囊包被缓释处理,研究了不同因素对缓释葡萄糖氧化酶酶活回收率和缓释作用的影响。结果显示,制备缓释葡萄糖氧化酶的最佳条件为:海藻酸钠浓度2.0%,壳聚糖浓度0.75%,葡萄糖氧化酶浓度0.3%,pH值5.0,钙离子浓度0.3mol/L,搅拌速度1200r/min。此时葡萄糖氧化酶酶活回收率最高,达到93.8%,且显示出较好的缓释性能。

关键词:  , 葡萄糖氧化酶, 壳聚糖/海藻酸钠, 缓释, 制备条件

Abstract: Chitosan /sodium alginat was selected as a compound wall material of controlled- release microcapsule of glucose oxidase in this study. Influence of different factors to the recovery of enzyme activity and the controlled-release process were investigated. The results showed that the optimal conditions to prepare the controlled- release glucose oxidase were concentra- tion of sodium alginate 2.0%,concentration of chitosan 0.75%,concentration of glucose oxidase 0.6%,pH 5.0,concentration of calcium ion 0.3%,stirring speed 1200r/min. Under this condition the recovery of enzyme activity reached 93.8%,and the controlled-release GOD showed better slow release character.

Key words: glucose oxidase, chitosan /sodium alginate, controlled-release, preparation condition