食品科学 ›› 2007, Vol. 28 ›› Issue (6): 243-245.

• 生物工程 • 上一篇    下一篇

药用真菌樟芝菌丝体多糖提取工艺及活性的研究

 刘华, 周堃, 贾薇, 张劲松, 潘迎捷   

  1. 上海农科院食用菌研究所; 上海水产大学食品科学学院; 上海水产大学食品科学学院 上海201106 南京农业大学生命科学院微生物系; 江苏南京210095; 上海200090; 上海201106;
  • 出版日期:2007-06-15 发布日期:2011-12-31

Extracting Conditions for Mycelia Polysaccharides of Antrodia camphorata and Its Activity

 LIU  Hua, ZHOU  Kun, JIA  Wei, ZHANG  Jin-Song, PAN  Ying-Jie   

  1. 1.Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2.Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; 3.College of Food Sciences, Shanghai Fisher University, Shanghai 200090, China
  • Online:2007-06-15 Published:2011-12-31

摘要: 本实验对樟芝菌丝体粗多糖的提取工艺进行了研究,通过L9(33)正交试验,研究了料液比、温度、提取时间对多糖提取率的影响,结果显示提取时间是影响多糖提取率的主要因素,最佳工艺为料液比1:30,温度90℃,时间2h,多糖提取率为10%。结合动物体外实验进行了免疫活性研究,确定6号样品能显著促进小鼠脾淋巴细胞的增殖。

关键词: 正交试验, 樟芝, 菌丝体多糖, 提取工艺

Abstract: The extracting conditions of mycelia polysaccharide from Antrodia camphorata were studied. The orthogonal test results showed that the optimum conditions are as follows: adding water at a ratio of 1:30 for dry mycelia to water, extracting for 2 h at 90 ℃ and the extraction rate of soluble polysaccharides 10%. The immunocompetence of different extracting conditions of polysaccharide were investigated with immunocyte cultivation technique in vitro. The experimental results showed that the proliferation of mouse lymphocytes is increased significantly by sample 6.

Key words: orthogonal design, Antrodia camphorata, mycelia polysaccharides, extracting condition