茶叶中多糖含量的测定

食品科学 ›› 2001, Vol. 22 ›› Issue (11): 69-73.

茶叶中多糖含量的测定

傅博强, 谢明勇, 聂少平, 周鹏, 王远兴

南昌大学功能食品与营养保健研究所

出版日期:2001-11-15 发布日期:2012-02-14

Method Simplified in Assaying Tea Polysaccharide

FU Bo-Qiang, Xie-Ming-Yong, Nie-Shao-Ping, Zhou-Peng, Wang-Yuan-Xing

Online:2001-11-15 Published:2012-02-14

摘要/Abstract

摘要： 茶样经80%乙醇回流除去单糖、双糖、低聚糖、茶多酚、氨基酸等杂质后用水提取,蒽酮-硫酸法比色测定。用精制茶多糖测得茶多糖对葡萄糖的换算因子。对江西婺源不同茶场茶叶中多糖的含量进行了测定,并与其它产地、品种的茶叶多糖含量进行了比较。研究结果表明,此测定方法简便,供试液在4h内显色稳定,重现性较好,平均回收率为99.2%±1.80%,RSD=1.82%(n=3)。江西婺源绿茶中多糖含量在4.17%～6.35%之间,CTC红碎茶多糖含量为3.10%～3.53%,其含量与茶叶品种、等级、生长海拔高度、产地有关。

关键词: , 茶叶多糖, 蒽酮-硫酸法, 含量测定, 婺源

Abstract: The interference components such as monosaccharide, disaccharide, oligosaccharide, tea polyphenol and amino acids in tea samples were removed by back- flowing with 80% ethanol. The contents of polysaccharide extracted with water in tea samples, which were collected from Wu- Yuan County of Jiangxi Province, were measured by anthrone- H2SO4 colorimetry, Meantime, compared with the polysaccharide contents in other kinds of tea. the results showed that the method used in this paper was simple, the color of the treated samples was stable in 4 h and the average value of the recovery for polysaccharide measurement was 99.2% ± 1.80% with 1.82% of RSD (n=3). The contents of polysaccharide in green tea of Wu- Yuan County ranged between 4.17% ? 6.35% and 3.10% ? 3.53% in CTC black tea. The different content might be related to the species, altitude, habitat and kind of tea grown.

Key words: Tea polysaccharide Anthrone- sulfuric acid method Determination Wu- Yuan County

傅博强, 谢明勇, 聂少平, 周鹏, 王远兴. 茶叶中多糖含量的测定[J]. 食品科学, 2001, 22(11): 69-73.

FU Bo-Qiang, Xie-Ming-Yong, Nie-Shao-Ping, Zhou-Peng, Wang-Yuan-Xing. Method Simplified in Assaying Tea Polysaccharide[J]. FOOD SCIENCE, 2001, 22(11): 69-73.

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