食品科学 ›› 2001, Vol. 22 ›› Issue (9): 62-64.

• 分析检测 • 上一篇    下一篇

灵芝菌生物活性多糖与总糖的测定

 苏亚玲,  程发良,  李纠,  阮湘元, 白燕   

  1. 东莞理工学院应用化学系; 暨南大学
  • 出版日期:2001-09-15 发布日期:2012-02-15

Determination Study on Glucose and Bioactive Poly saccharide in GLM

 SU  Ya-Ling,   Cheng-Fa-Liang,   Li-Jiu,   Ruan-Xiang-Yuan, BAI  Yan   

  • Online:2001-09-15 Published:2012-02-15

摘要: 以75%乙醇水溶液分离可溶性单糖和低聚糖,在3.5mol/LH2SO4溶液中和100℃下水解90min,使活性多糖(β-D-聚葡萄糖)水解成葡萄糖,然后用兰-埃农氧化还原滴定法滴定,终点颜色为浅棕色。较液相色谱法,不仅精密度明显高,而且测定值高出约四个百分点,更吻合实际组成。该方法生产商采用为生产工艺控制和产品质量认定的分析方法。

关键词: 灵芝菌, 生物活性多糖, 化学分析

Abstract: Samples of ganoderma lucidum mycelium(GLM)were treated with 75% alcohol solution to separate water- soluble saccharides from polysaccharide and then dissolved in 3.5mol/L H2SO4 solution under 100℃ for about 90min. and product,The glucose broken from bio- active polysaccharide in GLM.was determined with Land and Egnon’s Method based on oxidation- reduction titration. End point in the titration could be found when color of solution was suddenly changed from violet to light brown.

Key words: Ganoderma lucidum mycelium Bio- active polysaccharide Chemical analysis