北大西洋瓜参岩藻聚糖硫酸酯分离纯化及结构分析

食品科学 ›› 2012, Vol. 33 ›› Issue (19): 83-87.

北大西洋瓜参岩藻聚糖硫酸酯分离纯化及结构分析

于龙，薛长湖，宿玮，王彦超，张翠玉，常耀光*

中国海洋大学食品科学与工程学院

收稿日期:2011-07-31 修回日期:2012-08-14 出版日期:2012-10-15 发布日期:2012-09-17
通讯作者: 常耀光 E-mail:changyg@ouc.edu.cn
基金资助:
国际科技合作项目;冰提丝胶肽结构特性及抗冻作用机制的研究;海洋公益性行业科研专项

Purification and Characterization of Fucoidan Extracted from Cucumaria frondosa

Received:2011-07-31 Revised:2012-08-14 Online:2012-10-15 Published:2012-09-17

摘要/Abstract

摘要： 目的：从北大西洋瓜参(Cucumaria frondosa)体壁中提取纯化得到一种北大西洋瓜参岩藻聚糖硫酸酯(CF-FUC)，并对其结构特征进行研究。方法：利用阴离子交换层析和凝胶过滤层析纯化出CF-FUC组分，以高效凝胶过滤色谱法测定分子质量，以离子色谱法测定硫酸根含量，以柱前衍生高效液相色谱法测定单糖组成，并通过核磁共振技术推测其精细结构。结果：CF-FUC的分子质量为360kD，硫酸根含量为(29.8±3.2)%，单糖组成为岩藻糖、半乳糖、氨基葡萄糖、氨基半乳糖物质的量比为 1:0.22:0.07:0.07，岩藻糖存在两位硫酸酯化α型吡喃岩藻糖及非硫酸酯化α型吡喃岩藻糖两种形式。结论：CF-FUC结构有别于其他海参岩藻聚糖硫酸酯(SC-FUC)。

关键词: 海参, 北大西洋瓜参, 海参多糖, 岩藻聚糖硫酸酯, 结构

Abstract: In this study, fucoidan from Cucumaria frondosa body wall was purified and structurally characterized. A fraction named as CF-FUC was obtained by anion exchange chromatography and gel filtration chromatography. The fraction was analyzed for molecular weight by high-performance gel filtration chromatography, sulfate content by ion chromatography, monosaccharide composition by pre-column derivitization high-performance liquid chromatography, and fine structural characteristics by nuclear magnetic resonance. The molecular weight of CF-FUC was 360 kD with a sulfate content of (29.8 ± 3.2)%, and its monosaccharide composition included fucose, galactose, glucosamine and galactosamine at a ratio of 1:0.22:0.07:0.07. Moreover, NMR analysis revealed that the fucoidan was mainly composed of α-L-pFuc-2(OSO3) and α-L-pFuc. In conclusion, the structure of Cucumaria frondosa fucoidan is novel and different from that reported previously for sea cucumber fucoidan.

Key words: sea cucumber, Cucumaria frondosa, sea cucumber polysaccharide, fucoidan, structure

中图分类号:

TS254.1

于?龙，薛长湖，宿?玮，王彦超，张翠玉，常耀光*. 北大西洋瓜参岩藻聚糖硫酸酯分离纯化及结构分析[J]. 食品科学, 2012, 33(19): 83-87.

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