响应面法优化黑曲霉发酵低聚异麦芽糖培养基

摘要/Abstract

摘要： 采用全因子试验、最陡爬坡试验以及Box-Behnken试验设计对黑曲霉CU-1(Aspergillus niger CU-1)发酵生产低聚异麦芽糖培养基的主要成分进行优化。结果表明：最优培养基成分为：麸皮浸汁体积分数4%、玉米浆添加量19.67g/L、NaNO3添加量2.24g/L、木薯淀粉糖化液添加量250g/L，在该培养条件下，在3.6L发酵罐中进行验证，发酵液中异麦芽糖、潘糖和异麦芽三糖总产量达到37.4%，低聚异麦芽糖总产量高达83.1%，说明Box-Behnken试验设计法用于黑曲霉发酵生产低聚异麦芽糖培养基优化是可行的，数学模型的预测值与实验观察值相符。

关键词: 黑曲霉, α-葡萄糖苷酶, 响应面分析法, 培养基优化, 低聚异麦芽糖

Abstract: The major fermentation medium components for isomaltooligosaccharide production by Aspergillus niger CU-1 were optimized by full factorial, steepest ascent and Box-Behnken designs. The optimal medium components were determined to be 4% wheat bran extract, 19.67 g/L corn steep liquor, 2.24 g/L NaNO3, and 250 g/L saccharified cassava starch. Fermentation of the optimized medium by Aspergillus niger CU-1 was carried out in a 3.6 L fermenter to obtain isomaltose, panose and isomaltotriose yield as high as 37.4%, an isomaltooligosaccharide yield as high as 83.1%. The good agreement between the predictive and actual values indicated that the mathematical model established based on Box-Behnken design was valid.

Key words: Aspergillus niger, α-glucosidase, response surface methodology, medium optimization, isomaltooligosaccharides

中图分类号:

TS21 0

吴孔阳1，王学军2，周培华2，黄时海2，张云开2，陈桂光2，梁智群2,*. 响应面法优化黑曲霉发酵低聚异麦芽糖培养基[J]. 食品科学, 2012, 33(19): 216-222.

参考文献

[1]YUN J W, LEE M G, SONG S K.Continuous production of isomalto-oligosaccharides from maltose syrup by immobilized cells of permeabilized Aureobasidium pullulans[J].Biotechnol Lett,1994,16(11):1145-1150
[2]LIN Q, XIAO H, ZHAO J, et al.Production of isomalto-oligosaccharide syrup from rice starch using an one-step conversion method[J].International Journal of Food Science and Technology,2011,46(6):1194-1200
[3]LEE C C, WANG H F, LIN S D.Effect of isomaltooligosaccharide syrup on quality characteristics of sponge cake[J].Cereal Chemistry,2008,85(4):515-521
[4]LEE H S, AUH J H, YOON H G, et al.Cooperative action of alpha-glucanotransferase and maltogenic amylase for an improved process of isomaltooligosaccharide (IMO) production[J].Journal of Agricultural and Food Chemistry,2002,50:2812-2817
[5]GOULAS A K, COOPER J M, GRANDISON A S, et al..Synthesis of isomaltooligosaccharides and oligodextrans in a recycle membrane bioreactor by the combined use of dextransucrase and dextranase[J].,2004,88(6):778-787
[6]KURIKI T, YANASE M, TAKATA H, et al.A new way of producing isomalto-oligosaccharide syrup by using the transglycosylation reaction of neopullulanase[J].,1993,59(4):953-959
[7]ASLAN Y, TANRISEVEN A.Immobilization of Penicillium lilacinum dextranase to produce isomaltooligosacch- arides from dextran[J].Biochemical Engineering Journal,2007,34:8-12
[8]MINETOKI T, GOMI K, KITAMOTO K, et al.Nucleotide sequence and expression of alpha-glucosidase-encoding gene (agdA) from Aspergillus oryzae[J].Biosci Biotechnol Biochem,1995,59(8):1516-1521
[9]CHEN G G, LI W, ZHANG Y K, et al.A high-throughput method for screening of Aspergillus niger mutants with high transglycosylation activity by detecting non-fermentable reducing sugar[J].World Journal of Microbiology and Biotechnology,2011,27(6):1519-1523
[10]童星, 唐秋嵩, 吴玉飞,等.黑曲霉α-葡萄糖苷酶基因的克隆及其在毕赤酵母中的表达[J].微生物学报,2009,49(2):262-268
[11]陈朋, 韩跃武, 胡先望.α-葡萄糖苷酶菌株的选育及发酵条件的研究[J].工业微生物,2007,37(6):49-52
[12]WANG Y H, JIANG Y, DUAN Z Y, et al.Expression and characterization of an α-glucosidase from Thermoanaerobacter ethanolicus JW200 with potential for industrial application[J].Biologia,2009,64(6):1053-1057
[13]CHEN D L, TONG X, CHEN S W, et al.Heterologous expression and biochemical characterization of α-glucosidase from Aspergillus niger by Pichia pastroris[J].Journal of Agricultural and Food Chemistry,2010,58(8):4819-4824
[14]ZHOU C, XUE Y, ZHANG Y, et al.Recombinant expression and characterization of Thermoanaerobacter tengcongensis thermostable alpha-glucosidase with regioselectivity for high yield isomaltooligosaccharides synthesis[J].,2009,19(12):1547-1556
[15]ZHANG L, JIANG Y, JIANG Z, et al.Immobilized transglucosidase in biomimetic polymer–inorganic hybrid capsules for efficient conversion of maltose to isomaltooligosaccharides[J].Biochemical Engineering Journal,2009,46:186-192
[16]XU Y X, LI Y L, XU S C, et al.Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition[J].,2008,9(7):558-566
[17]LI J, DING S, DING X.Optimization of the ultrasonically assisted extraction of polysaccharides from Zizyphus jujuba cvjinsixiaozao[J].,2007,80(1):176-183
[18]曾文炉, 赵飞飞, 曹照根,等.利用响应面方法优化转小鼠金属硫蛋白-I基因聚球藻7002的培养基成分[J].生物工程学报,2008,24(1):130-136
[19]BARRINGTON S, KIM J.Response surface optimization of medium components for citric acid production by Aspergillus niger NRRL 567 grown in peat moss[J].Bioresource Technology,2008,99(2):368-377
[20]LUDWIG T G, GOLDBERG H J V.The Anthrone Method for the Determination of Carbohydrates in Foods and in Oral Rinsing[J].Journal of Dental Research,1956,35(1):90-94
[21]JERMYN M A.Increasing the sensitivity of the anthrone method for carbohydrate[J].Analytical Biochemistry,1975,68(1):332-335
[22]MAITI B, RATHORE A, SRIVASTAVA S, et al.Optimization of process parameters for ethanol production from sugar cane molasses by Zymomonas mobilis using response surface methodology and genetic algorithm[J].Applied Microbiology and Biotechnology,2011,90(1):385-395
[23]郭洁, 洪子雯, 方晓玲.Box-Behnken实验设计法优化表阿霉素脂质体的处方工艺[J].复旦学报（医学版,2007,34(6):816-820
[24]LEE S L, CHEN W C.Optimization of medium composition for the production of glucosyltransferase by Aspergillus niger with response surface methodology[J].Enzyme and Microbial Technology,1997,21(6):436-440
[25]YAN T R, LIU D.Purification and characterization of a transglucosidase from Aspergillus niger CCRC31494[J].J. Chin.Biochem. Sot,1993,22:55-67
[26]HANG Y D, WOODAMS E E.Enzymatic production of panose from maltose by glucosyltransferase from Aspergillus foetidus[J].,1995,17(12):1335-1336
[27]GOKHALE D V, PATIL S G, BASTAWDE K B.Optimization of cellulase production by Aspergillus niger NCIM 1207[J].Applied Biochemistry and Biotechnology,1991,30(1):99-109
[28]EL-GINDY A A. U,F. ALI Z M I, ISAAC G S.A cost-effective medium for enhanced production of extracellular α-galactosidase in solid Substrate cultures of Aspergillus awamori and A. carbonarius[J].Australian Journal of Basic and Applied Sciences,2008,2(4):880-899
[29]OBAYORI O S, ADEBUSOYE S A, ILORI M O, et al.Effects of Corn Steep Liquor on Growth Rate and Pyrene Degradation by Pseudomonas strains[J].,2009,60(6):407-411