黑曲霉降酸菌株F1降解酒石酸关键酶的分离纯化

食品科学 ›› 2012, Vol. 33 ›› Issue (15): 235-238.

黑曲霉降酸菌株F1降解酒石酸关键酶的分离纯化

王贵珍，董昕，文连奎*

吉林农业大学食品科学与工程学院

收稿日期:2011-09-25 修回日期:2012-07-17 出版日期:2012-08-15 发布日期:2012-09-07
通讯作者: 文连奎 E-mail:wenliankui@163.com
基金资助:
山葡萄酒中生物降解酒石酸关键技术与产业化开发

Isolation and Purification of Key Tartaric Acid Degrading Enzyme from Aspergillus niger F1

Lian-kui WEN

Received:2011-09-25 Revised:2012-07-17 Online:2012-08-15 Published:2012-09-07
Contact: Lian-kui WEN E-mail:wenliankui@163.com

摘要/Abstract

摘要： 为得到黑曲霉菌株F1中具有降酸作用的蛋白，将黑曲霉菌株F1经酒石酸诱导发酵得到菌丝体，用液氮研磨破碎细胞壁，超声波辅助提取得到粗酶液，硫酸鱼精蛋白去除核酸，热变除杂蛋白，聚乙二醇浓缩，透析脱盐，DEAE-纤维素52阴离子交换层析，Sephadex G-75葡聚糖凝胶柱分子筛层析，最终得到该蛋白的单一组分，其纯化倍数为14.32，并确定该酶为脱氢酶类。

关键词: 黑曲霉, 酒石酸降解酶, 分离纯化

Abstract: The purpose of this study was to prepare a key tartaric acid degrading enzyme from Aspergillus niger F1. Aspergillus niger F1 was cultured and the expression of tartaric acid degrading enzymes was induced by adding tartaric acid into the medium. Cultured mycelia of Aspergillus niger F1 were collected and homogenized in liquid nitrogen. Crude enzyme extract was obtained by ultrasonic assisted extraction, added with protamine sulfate for removing nucleic acids, denatured for removing unwanted proteins, concentrated by PEG treatment, dialyzed for desalting, and then purified by DEAE-cellulose 52 anion exchange chromatography and Sephadex G-75 gel filtration chromatography to obtain a single component with a purification factor of 14.32. The enzyme was proven to be a dehydrogenase.

Key words: Aspergillus niger, tartaric acid degrading enzymes, separation and purification

中图分类号:

Q936

王贵珍，董昕，文连奎* . 黑曲霉降酸菌株F1降解酒石酸关键酶的分离纯化[J]. 食品科学, 2012, 33(15): 235-238.

Lian-kui WEN. Isolation and Purification of Key Tartaric Acid Degrading Enzyme from Aspergillus niger F1[J]. FOOD SCIENCE, 2012, 33(15): 235-238.

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