小蓟中甾体类化合物的分离及结构鉴定

食品科学 ›› 2012, Vol. 33 ›› Issue (19): 124-127.

小蓟中甾体类化合物的分离及结构鉴定

孙珍，李泠鸰，院珍珍，王阿利，李金杰，尚小雅*

北京联合大学生物活性物质与功能食品北京市重点实验室

收稿日期:2012-06-08 修回日期:2012-09-03 出版日期:2012-10-15 发布日期:2012-09-17
通讯作者: 孙珍 E-mail:sunzhensdcx@126.com
基金资助:
北京市教委面上项目资助课题

Isolation, Purification and Structural Elucidation of Steroids from Cirsium setosum (Willd.) MB

Received:2012-06-08 Revised:2012-09-03 Online:2012-10-15 Published:2012-09-17

摘要/Abstract

摘要： 对小蓟乙醇粗提物中显示细胞毒活性的石油醚萃取部位进行化学成分的研究。运用硅胶、凝胶和反相高效液相等色谱方法进行分离纯化；应用一维、二维NMR和MS等波谱学方法对纯化的物质进行结构鉴定。结果表明：从小蓟乙醇提取物的石油醚萃取部位分离鉴定了4个甾体类化合物，分别为：麦角甾-4,24(28)-二烯-3-酮(化合物1)、豆甾-4-烯-3-酮(化合物2)、豆甾醇(化合物3)和β-谷甾醇(化合物4)。化合物1和2为首次从蓟属中分离得到，除化合物4外，其余3个化合物均是首次从小蓟中分离得到；在体外MTT法的细胞毒活性测试中，所筛选的化合物在1×10-5mol/L的浓度条件下均未显示出细胞毒活性。

关键词: 小蓟, 分离纯化, 结构鉴定, 甾体, 细胞毒活性

Abstract: The petroleum ether-soluble fraction of pooled ethanol extracts from the aboveground parts of Cirsium setosum (Willd.) MB was further fractionated by silica gel column chromatography, Sephadex LH-20 column chromatography and preparative HPLC. The resulting sub-fractions were structurally identified by one-dimensional, two-dimensional NMR and MS spectroscopies. Four steroids were obtained, including ergosta-4, 24 (28)-diene-3-one (1), stigmast-4-en-3-one (2), stigmasterol (3) and β-sitosterol (4). To the best of our knowledge, this is the first report showing the presence of Compounds 1 and 2 in the genus Cirsium and the presence of Compounds 1, 2 and 3 in Cirsium setosum (Willd.) MB. None of these identified compounds showed cytotoxic activity at a concentration of 1 × 10-5 mol/L as determined by in vitro MTT assay.

Key words: Cirsium setosum, isolation and purification, structural elucidation, steroids, cytotoxicity

中图分类号:

Q74
R914

孙?珍，李泠鸰，院珍珍，王阿利，李金杰，尚小雅* . 小蓟中甾体类化合物的分离及结构鉴定[J]. 食品科学, 2012, 33(19): 124-127.

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