Enterocin A构效关系的研究

摘要/Abstract

摘要： 通过基因技术对Enterocin A的第14位半胱氨酸、第46位赖氨酸、第47位半胱氨酸分别进行丝氨酸、谷氨酸或谷氨酰胺、色氨酸替换，利用大肠杆菌表达系统获得MBP-Enterocin A氨基酸替换突变体。以伊氏李斯特氏菌LIV 2为指示菌，抗菌活性结果表明：赖氨酸替换突变体MBP-Enterocin A(K46E)或MBP-Enterocin A(K46Q)具有与MBP-Enterocin A相当的抗菌活性，而半胱氨酸替换突变体MBP-Enterocin A (C14S)或MBP-Enterocin A (C47W)均不具有抗菌活性。因此，初步判定 K46 不是Enterocin A抗菌活性的关键氨基酸，而C14 、C47为Enterocin A的关键氨基酸。

关键词: Enterocin A, 突变体, 抗李斯特菌活性

Abstract: In order to analyze the relationship between molecular structure and antilisterial activity of enterocin A, MBP-enterocin A mutants (C14S, K46E or K46Q, and C47W) with serine, glutamic acid or glutamine, and tryptophane as a substitute for cysteine (at position 14), lysine (at position 46) and cysteine (at position 47) respectively were created and expressed in Escherichia coli fusion expression systems. The mutants K46E and K46Q showed similar anti-Listeria ivanovii LIV2 activity to MBP-enterocin A, while the mutants C14S and C47W had no inhibitory effect on Listeria ivanovii LIV2. Therefore, the residues C14 and C47 rather than K46 were essential for the antibacterial function of enterocin A.

Key words: enterocin A, mutant, antilisterial activity

中图分类号:

TS201.3

赵爱珍，徐兴然. Enterocin A构效关系的研究[J]. 食品科学, 2012, 33(19): 269-272.

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