食品科学 ›› 2013, Vol. 34 ›› Issue (2): 72-77.

• 工艺技术 • 上一篇    下一篇

水酶法从巴旦木中提油及水解蛋白的研究

孙月娥   

  1. 徐州工程学院食品学院
  • 收稿日期:2012-06-23 修回日期:2012-12-27 出版日期:2013-01-25 发布日期:2013-01-15
  • 通讯作者: 孙月娥 E-mail:sunyuee416@yahoo.com.cn

Aqueous enzymatic extraction of badanmu oil and protein hydrolysate

  • Received:2012-06-23 Revised:2012-12-27 Online:2013-01-25 Published:2013-01-15

摘要: 采用水酶法从巴旦木中同时提取油与水解蛋白。依次使用复合细胞壁多糖水解酶(纤素酶:果胶酶=1:2)和碱性蛋白酶水解巴旦木浆,并对酶解工艺条件进行优化。通过单因素试验及正交试验,确定了水酶法提取巴旦木油的最佳工艺条件:料液比1:5、粒径40目、复合细胞壁多糖水解酶用量3.5%、酶解温度40℃、酶解时间4h、碱提pH 9.0、蛋白酶用量1.5%、酶解温度50℃、酶解时间2h;水酶法提取巴旦木水解蛋白的最佳工艺:料液比1:5、粒径30目、细胞多糖水解酶用量3%、提取温度50℃、提取时间3h、碱提pH 8.5、蛋白酶用量1.5%、酶解温度50℃、酶解时间2.5h。在此最佳条件下进行试验验证,总的巴旦木游离油和水解蛋白得率分别为68.74% 和 74.39%。

Abstract: The aqueous enzymatic process of simultaneously preparing oil and protein hydrolysates from BaDanMu was introduced. Badanmu slurry was treated with complex cell-wall polysaccharides degrading enzymes (cellulase:pectinase = 1:2) and Alcalase sequentially, and the optimal conditions for enzymatic hydrolysis were investigated. Through single factor experiment and the orthogonal experiment, the optimum conditions for extraction of badanmu oil are as follows: solid-liquid ratio is 1:5, particle size 30 mesh, cell polysaccharide hydrolysis enzyme content is 3.5%, extraction temperature 40 ℃, extracting time 4 h, alkali extraction pH 9, protease content is 1.5%, 50 ℃ temperature enzyme solution, enzymatic hydrolysis time 2 h;the optimum conditions for extraction of badanmu hydrolyzed protein: solid-liquid ratio is 1:5, particle size 30 mesh, cell polysaccharide hydrolysis enzyme content is 3%, 50 ℃, extraction temperature extracting time 3 h, alkali mention pH 8.5, protease content is 1.5%, 50 ℃ temperature enzyme solution, enzymatic hydrolysis time 2.5 h. Under the optimum conditions, the total extraction rates of free oil and protein hydrolysates are 68.74% and 74.39%, respectively.

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