食品科学 ›› 2014, Vol. 35 ›› Issue (2): 87-90.doi: 10.7506/spkx1002-6630-201402016

• 工艺技术 • 上一篇    下一篇

超声波辅助酶法分离提取葡萄酒泥酵母SOD工艺条件的优化

杜 娜1,杨学山2,*,韩舜愈1,祝 霞1,付文力2,王 婧1,盛文军1,张 波1   

  1. 1.甘肃农业大学食品科学与工程学院,甘肃 兰州 730070;2.甘肃农业大学生命科学技术学院,甘肃 兰州 730070
  • 收稿日期:2013-01-05 修回日期:2013-12-24 出版日期:2014-01-25 发布日期:2014-02-19
  • 通讯作者: 杨学山 E-mail:yangxs@gsau.edu.cn
  • 基金资助:

    甘肃省科技支撑计划项目(1104NKCA080)

Optimization of Process Conditions for Ultrasonic-Assisted Enzymatic Extraction of Superoxide Dismutase from Waste Wine Yeast

DU Na1, YANG Xue-shan2,*, HAN Shun-yu1, ZHU Xia1, FU Wen-li2, WANG Jing1, SHENG Wen-jun1, ZHANG Bo1   

  1. 1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;
    2. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2013-01-05 Revised:2013-12-24 Online:2014-01-25 Published:2014-02-19
  • Contact: YANG Xue-shan E-mail:yangxs@gsau.edu.cn

摘要:

以葡萄酒泥酵母为试材,超氧化物歧化酶(SOD)比活力为指标,在超声功率、超声时间、蜗牛酶质量浓 度、酶解pH值和酶解温度等单因素试验基础上,采用正交试验优化超声波辅助蜗牛酶法提取SOD的工艺条件。结果 表明:提取工艺因素对SOD比活力影响大小顺序为超声时间>蜗牛酶质量浓度>超声功率>酶解pH值>酶解温度, 1 g湿酵母悬浮于5 mL 0.2 mol/L的磷酸盐缓冲液,其提取SOD最佳工艺条件为超声功率400W、超声时间12 min、 2.0 mg/100 mL蜗牛酶液1 mL、pH 6.6、温度37 ℃,在此条件下,分离提取得到的SOD比活力达192.27 U/mg。结果表 明超声波辅助酶法在葡萄酒泥酵母SOD的提取中具有较高的应用价值。

关键词: 葡萄酒泥酵母, 超氧化物歧化酶, 超声波, 蜗牛酶

Abstract:

The current work was designed to establish an optimal procedure for the extraction of superoxide dismutase (SOD)
from waste wine yeast by ultrasonic-assisted snailase hydrolysis. Five process parameters, ultrasonic power, radiation time,
enzyme concentration, pH and temperature were optimized by one-factor-at-a-time and orthogonal array design. The effects
of these variables on SOD specific activity in extracts decreased in this order: radiation time > enzyme concentration >
ultrasonic power > pH > hydrolysis time. The optimal extraction conditions were found to be ultrasonic pretreatment at a
power of 400 W for 12 min and subsequent hydrolysis with 1 mL of 2.0 mg/100 mL snailase solution at 37 ℃ with an initial
pH of 6.6. Under these optimal conditions, the specific activity of SOD was 192.27 U/mg. The ultrasonic-assisted snailase
hydrolysis procedure deserves further application in the production of SOD from waste wine yeast.

Key words: wine yeast, superoxide dismutase (SOD), ultrasound, snailase

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