食品科学

• 基础研究 • 上一篇    下一篇

普洱茶提取物主要活性成分及抗氧化作用

陈玉琼,樊 蓉,刘思思,倪德江   

  1. 华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,湖北 武汉 430070
  • 出版日期:2013-10-15 发布日期:2013-09-27
  • 通讯作者: 陈玉琼

Phenolic Composition and Antioxidant Activity of Pu-erh Tea Extracts

CHEN Yu-qiong,FAN Rong,LIU Si-si,NI De-jiang   

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences,
    Huazhong Agricultural University, Wuhan 430070, China
  • Online:2013-10-15 Published:2013-09-27
  • Contact: CHEN Yu-qiong

摘要:

用乙酸乙酯、正丁醇萃取普洱茶水提液,分析其主要活性成分及抗氧化作用。结果表明:儿茶素在各组分中含量依次为:乙酸乙酯组分(73.79%)>正丁醇组分(34.93%)>水提取组分(32.49%)>剩余水层(13.54%);茶黄素、没食子酸主要在乙酸乙酯组分中,正丁醇组分含较多茶红素。普洱茶水提物具有一定抗氧化作用,其中,乙酸乙酯组分表现出较强的O2-•清除活性,而剩余水层则表现出较强的•OH清除活性。前者起作用的机制可能与没食子酸、儿茶素和茶黄素等活性成分有关,后者可能与茶褐素等其他物质有关。因此,普洱茶中含有分别具有O2-•、•OH清除作用的成分,控制普洱茶发酵过程可能会获得具有不同自由基清除作用的活性成分。

关键词: 普洱茶, 萃取组分, 化学成分, 抗氧化性

Abstract:

The components of Pu-erh ethyl acetate extract (PEE), butyl alcohol extract (PBE) and residual water extract
(PRWE) from Pu-erh tea water extract (PWE) and their antioxidant activities were studied. The results showed that the content
of total catechins followed the order: PEE (73.79%) > PBE (34.93%) > PWE (32.49%) > PRWE (13.54%), and that aflavins
and gallic acid were mainly concentrated in PEE, except for thearubigin in PBE. PWE had strong antioxidant activity. PEE and
PRWE exhibited the most powerful scavenging activity against O2
–• and •OH, respectively. The possible mechanism for the
scavenging effect of Pu-erh tea extracts against •OH seemed different from O2
–•. The former could be caused by multiple active
factors such as GA, catechins and TF, but the latter by TB or other substances. It is possible to obtain functional Pu-erh tea
containing active factors against different free radicals (especially •OH) via controlling the fermentation process.

Key words: Pu-erh tea, extracts, chemical components, antioxidant action

中图分类号: