• 成分分析 •

### 双缩脲法测定罗非鱼源胶原蛋白肽含量的改良及应用评价

1. （1.华南理工大学食品科学与工程学院，广东 广州 510641；2.广东石油化工学院生物与食品工程学院，广东 茂名 525000）
• 出版日期:2019-10-25 发布日期:2019-10-25
• 基金资助:
广东省科技计划项目（2015B020230001）；广东普通高校食品科学创新团队项目（2016KCXTD020）； 广东石油化工学院2018年大学生创新创业校级培训计划项目（2018pyA038）

### ZHANG Ling, SHI Caiwen, XIAO Kaijun, LI Chunhai, MO Junquan, ZHANG Zhong, SHI Yuyu

ZHANG Ling, SHI Caiwen, XIAO Kaijun, LI Chunhai, MO Junquan, ZHANG Zhong, SHI Yuyu

1. (1. School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China;2. College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China)
• Online:2019-10-25 Published:2019-10-25

Abstract: To develop a rapid method for the determination of tilapia collagen peptide content, the colorimetric biuret method was improved using tilapia skin collagen peptide as a reference material. We determined the maximum absorption wavelength of the chromogenic complex and the concentration ranges of peptide and trichloroacetic acid (TCA) and pH for high linearity. The repeatability, precision and recovery of the improved method were tested under optimized conditions. The applicability of this method to the detection of collagen peptides from the skin and scales of tilapia with different molecular masses was also studied. The results showed that the absorbance was linearly fitted with peptide concentration ranging from 0.3 to 1.5 mg/mL under the following conditions: 13% TCA, adjustment of the system pH to 12.5, and detection wavelength 545?nm, and the following equation was obtained: y = 0.144 9 x + 0.001 5 (R2 = 0.999). The relative standard deviation (RSD) for repeatability and precision were 1.86% and 1.84%, respectively. The average spiked recoveries with and without the addition of gelatin were 113.9% and 109.7%, with RSD of 1.39% and 1.00%, respectively. The method had wide linear range, good repeatability and high accuracy. Its application to different tilapia collagen peptides showed little differences in spiked recoveries and RSD. The improved biuret method proved to be simple, rapid and suitable for the determination of peptides derived from tilapia.