关键词: 橄榄；果实；采后病害；潜伏侵染；小新壳梭孢；小孢拟盘多毛孢

Abstract: In order to clarify the species of latent pathogenic fungi in Chinese olive fruit, the pathogenic fungi from the flower organ and fruit of ‘Changying’ Chinese olive (Canarium album (Lour.) Raeusch cv. Changying) were isolated by using tissue isolation, and their pathogenicity was assayed using Koch’s postulates. The genomic (DNA of the isolated pathogens was extracted via the cetyl trimethyl ammonium bromide (CTAB) method Polymerase chain reaction (PCR) was employed to amplify of the ribosomal DNA internal transcribed spacer (rDNA-ITS) region, and then the amplified product was sequenced and subjected to homology analysis by basic local alignment search tool (BLAST). A phylogenetic tree for each of the six isolates was established using the neighbor-joining (NJ) method with MEGA6.0 software. According to their morphological and phylogenetic characteristics, the six latent pathogenic fungi were identified as Neofusicoccum parvum, Pestalotiopsis microspora, Aureobasidium pullulans, Phomopsis, Phanerochaete, and Guignardia with N. parvum and P. microspora being the dominant ones, which could infect the plant at the blooming stage. The results of this work can provide a scientific basis for controlling postharvest disease of Chinese olive fruit.

Key words: Chinese olive; fruit; postharvest disease; latent infection; Neofusicoccum parvum; Pestalotiopsis microspora