食品科学 ›› 2020, Vol. 41 ›› Issue (18): 288-295.doi: 10.7506/spkx1002-6630-20190603-014

• 安全检测 • 上一篇    下一篇

基于变性血红蛋白和二胺氧化酶的生物传感器检测马鲛鱼中的尸胺

张敏,吴俊铨,姚嘉文,杨春婷,白卫东,赵晓娟   

  1. (1.仲恺农业工程学院轻工食品学院,广东?广州 510225;2.广州市广式传统食品加工与安全控制重点实验室,广东?广州 510225)
  • 出版日期:2020-09-25 发布日期:2020-09-18
  • 基金资助:
    广东省科技计划项目(2016A040403116);广州市科技计划项目(201604020021); “十三五”国家重点研发计划重点专项(2018YFD0901003)

Determination of Cadaverine in Mackerel by Biosensor Based on Unfolded Hemoglobin and Diamine Oxidase

ZHANG Min, WU Junquan, YAO Jiawen, YANG Chunting, BAI Weidong, ZHAO Xiaojuan   

  1. (1. College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; 2. Guangzhou Key Laboratory of Traditional Cantonese Food Processing and Safety Control, Guangzhou 510225, China)
  • Online:2020-09-25 Published:2020-09-18

摘要: 通过物理吸附法将变性血红蛋白(unfolded hemoglobin,uHb)固定在黏土-纳米金复合材料(Clay-AuNPs)修饰的玻碳电极表面,然后以2.5%的戊二醛溶液为交联剂固定二胺氧化酶(diamine oxidase,DAO),构建一种对尸胺有良好检测性能的电化学生物传感器。对电极修饰体系、DAO浓度、过氧化物酶(模拟酶)的类型、修饰电极的稳定性及测试条件进行优化,通过循环伏安法和电化学交流阻抗法进行表征,并用于马鲛鱼中尸胺含量的检测。结果表明:在优化条件下该传感器的响应电流和尸胺浓度在2.0×10-12~1.0×10-11?mol/L有良好的线性关系(r=0.991),检出限(RSN=3)为6.7×10-13?mol/L。该生物传感器法测定马鲛鱼中尸胺的加标回收率为82.2%~109.1%。该法具有良好的重复性和稳定性,且灵敏度高,适用于马鲛鱼等样品中尸胺含量的测定。

关键词: 尸胺;生物传感器;二胺氧化酶;纳米金;马鲛鱼

Abstract: Unfolded hemoglobin was immobilized onto the surface of a clay-gold nanoparticles (Clay-AuNPs) modified glassy carbon electrode (GCE) by the physical adsorption method, and then diamine oxidase (DAO) was immobilized with 2.5% glutaraldehyde as a crosslinking agent. The resulting electrochemical biosensor had good detection performance for cadaverine. The electrode modification system, DAO concentration, the type of mimic peroxidase, the stability of the modified electrode and the test conditions were optimized. The modified electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Furthermore, the method was used to detect the cadaverine content in mackerel. The results showed that under the optimized conditions, the response current of the sensor had a good linear relationship with cadaverine concentration in the range between 2.0 × 10-12 and 1.0 × 10-11 mol/L (r = 0.991), and the detection limit (RSN = 3) was 6.7 × 10-13 mol/L. The spiked recoveries of cadaverine in mackerel samples by this biosensor method ranged from 82.2% to 109.1%. Thanks to its good reproducibility and stability and high sensitivity, this method is suitable for the determination of cadaverine in fish samples such as mackerel.

Key words: cadaverine; biosensors; diamine oxidase; gold nanoparticles; mackerel

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