裸脚菇0612-9活性物质对柑橘青、绿霉病的生防效果及其诱导抗性

doi:10.7506/spkx1002-6630-20191205-064

食品科学 ›› 2021, Vol. 42 ›› Issue (1): 272-278.doi: 10.7506/spkx1002-6630-20191205-064

裸脚菇0612-9活性物质对柑橘青、绿霉病的生防效果及其诱导抗性

何甜，崔朝宇，郑先能，龙昊知，霍光华

（江西农业大学江西省菌物资源保护与利用重点实验室，江西南昌 330045）

发布日期:2021-01-18
基金资助:
江西省自然科学基金项目（20171ACB20014）；国家自然科学基金地区科学基金项目（31560574）；江西省现代农业食用菌产业技术体系专项（JXARS-20）

Biocontrol of Blue and Green Molds and Induction of Disease Resistance in Citrus Fruit by Bioactive Substances from Gymnopus sp. 0612-9

HE Tian, CUI Chaoyu, ZHENG Xianneng, LONG Haozhi, HUO Guanghua

(Jiangxi Key Laboratory of Conservation and Utilization for Fungal Resources, Jiangxi Agricultural University, Nanchang 330045, China)

Published:2021-01-18

摘要/Abstract

摘要： 为研究裸脚菇0612-9活性物质对柑橘青、绿霉病的生防效果及其诱导抗性，以‘南丰’蜜桔为试材，采用质量浓度分别为0（对照）、0.25、0.55、5.50 mg/mL的裸脚菇0612-9发酵液粗提物（extract of fermentation broth from Gymnopus sp. 0612-9，GSFE）通过损伤接种的方式处理果实，随后分别接种青、绿霉病原菌孢子悬浮液，定期统计果实发病率，并取样测定柑橘果皮防御酶活力等指标的变化。结果表明：不同质量浓度GSFE处理较对照组均能显著降低柑橘青、绿霉病发病率及病情指数（P＜0.05），其中GSFE质量浓度为5.50 mg/mL时的抑制效果最佳；同时，GSFE处理能够诱导‘南丰’蜜桔果皮中相关防御酶（苯丙氨酸解氨酶、超氧化物歧化酶、过氧化物酶）活力的提高，促进其抗氧化物质总酚和类黄酮的产生，并抑制脂氧合酶活力、减少丙二醛的积累、降低膜脂过氧化程度，从而提高‘南丰’蜜桔果实的抗病性。本研究结果可为设计开发GSFE类新型柑橘采后防腐保鲜剂提供思路。

关键词: 裸脚菇0612-9发酵液粗提物；诱导抗性；意大利青霉；指状青霉；生物防治

Abstract: This study was undertaken in order to investigate the biocontrol effect of the ethyl acetate extract of the fermentation broth of Gymnopus sp. 0612-9 (GSFE) on blue and green molds in ‘Nanfeng’ citrus and to evaluate the efficacy in inducing disease resistance. Citrus fruit were pre-treated with different concentrations (0, 0.25, 0.55 and 5.50 mg/mL) of GSFE, and then artificially wounded and inoculated with spore suspensions of blue and green molds. Subsequently, the infection rate was quantified and changes in peel defense enzyme activities were measured regularly. The results indicated that the infection rate and disease index were both significantly reduced by GSFE treatment at all concentrations tested when compared with the control group, being the most effective at a concentration of 5.50 mg/mL. Moreover, the activities of phenylalanine ammonialyase, superoxide dismutase, peroxidase were enhanced remarkably by GSFE treatment. GSFE increased significantly the contents of total phenolics and total flavonoids, decreased the accumulation of malondialdehyde (MDA) and lipoxygenase (LOX) activity, and reduced membrane lipid peroxidation, thereby improving disease resistance. This study provides an idea for the development of GSFE as a postharvest citrus preservative.

Key words: extract of the fermentation broth of Gymnopus sp. 0612-9; induction of disease resistance; Penicillium italicum; Penicillium digitatum; biocontrol

中图分类号:

TS255.36

何甜，崔朝宇，郑先能，龙昊知，霍光华. 裸脚菇0612-9活性物质对柑橘青、绿霉病的生防效果及其诱导抗性[J]. 食品科学, 2021, 42(1): 272-278.

HE Tian, CUI Chaoyu, ZHENG Xianneng, LONG Haozhi, HUO Guanghua. Biocontrol of Blue and Green Molds and Induction of Disease Resistance in Citrus Fruit by Bioactive Substances from Gymnopus sp. 0612-9[J]. FOOD SCIENCE, 2021, 42(1): 272-278.

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裸脚菇0612-9活性物质对柑橘青、绿霉病的生防效果及其诱导抗性

Biocontrol of Blue and Green Molds and Induction of Disease Resistance in Citrus Fruit by Bioactive Substances from Gymnopus sp. 0612-9

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