食品科学 ›› 2021, Vol. 42 ›› Issue (5): 215-220.doi: 10.7506/spkx1002-6630-20200304-057

• 包装贮运 • 上一篇    下一篇

臭氧对杏果实黑斑病的抑制及贮藏保鲜作用

李玲,李智,石玲,李亚玲,何欢,张亚琳,芦玉佳,朱璇   

  1. (新疆农业大学食品科学与药学学院,新疆 乌鲁木齐 830052)
  • 出版日期:2021-03-15 发布日期:2021-03-29
  • 基金资助:
    国家自然科学基金地区科学基金项目(31860462);南京农业大学-新疆农业大学联合基金项目(KYYJ201908)

Inhibitory Effect and Mechanism of Ozone on the Black Spot Disease of Apricot Fruit

LI Ling, LI Zhi, SHI Ling, LI Yaling, HE Huan, ZHANG Yalin, LU Yujia, ZHU Xuan   

  1. (College of Food Science and Pharmacy, Xinjiang Agricultural University, ürümqi 830052, China)
  • Online:2021-03-15 Published:2021-03-29

摘要: 为了研究臭氧对杏果实采后黑斑病的抑制效果,以新疆‘赛买提’杏为试材,损伤并接种链格孢菌(Alternaria alternata),接种后的杏果实用200 mg/m3臭氧气体进行熏蒸处理,每次熏蒸30 min,每7 d处理一次,处理后的接种链格孢菌杏果实置于1~2 ℃、90%~95%相对湿度条件下贮藏49 d,定期统计其病斑直径和接种发病率,并测定苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)、肉桂酸-4-羟化酶(cinnamic acid-4-hydroxylase,C4H)、4-香豆酸辅酶A连接酶(4-coumaric acid CoA ligase,4CL)、β-1,3-葡聚糖酶(β-1,3-glucanase,GLU)和几丁质酶(chitinase,CHT)活力及总酚和类黄酮含量。结果表明:与对照相比,200 mg/m3臭氧可有效提高杏果实PAL、C4H、4CL、GLU和CHT活力,增加杏果实总酚和类黄酮含量,显著抑制杏果实病斑直径和接种发病率的增加(P<0.05),增强杏果实对黑斑病的抗病性。说明臭氧处理增强杏果实的抗病性与调控苯丙烷代谢密切相关。

关键词: 杏果实;臭氧;采后病害;苯丙烷代谢

Abstract: In order to study the effect of ozone on controlling the postharvest black spot disease of apricot fruit, freshly harvested ‘Saimaiti’ apricot fruit grown in Xinjiang, China were artificially wounded, inoculated with Alternaria alternate, fumigated with 200 mg/m3 of ozone for 30 minutes, and stored at 1–2 ℃ and 90%–95% relative humidity for 49 days. During this period, fumigation was repeated once every seven days for 42 days. The lesion diameter and disease incidence of apricot fruit inoculated with A. alternata, the activities of phenylalanine ammonia-lyase (PAL), cinnamic acid-4-hydroxylase (C4H), 4-coumaric acid CoA ligase (4CL), β-1,3-glucanase (GLU) and chitinase (CHT), and the total phenolic and flavonoid contents were measured regularly. The results showed that ozone treatment increased the activities of PAL, C4H, 4CL, GLU and CHT and the contents of total flavonoids and total phenolics in apricot fruit. Besides, ozone treatment significantly inhibited lesion expansion and the increase in the incidence of black spot disease (P < 0.05), and improved the disease resistance of apricot fruit by a mechanism closely related to the regulation of phenylpropane metabolism.

Key words: apricot fruit; ozone; postharvest diseases; phenylpropane metabolism

中图分类号: