食品科学 ›› 2021, Vol. 42 ›› Issue (10): 52-57.doi: 10.7506/spkx1002-6630-20210118-187

• 食品化学 • 上一篇    下一篇

豆渣可溶酸性多糖的分离纯化及结构解析

王思琪,胡彦波,翟丽媛,石曾卉,赵珺   

  1. (长春大学食品科学与工程学院,吉林 长春 130022)
  • 出版日期:2021-05-25 发布日期:2021-06-02
  • 基金资助:
    吉林省科技厅重点研发项目(20200402061NC)

Extraction, Purification and Structure Analysis of Soluble Acidic Polysaccharides from Soybean Dregs

WANG Siqi, HU Yanbo, ZHAI Liyuan, SHI Zenghui, ZHAO Jun   

  1. (College of Food Science and Engineering, Changchun University, Changchun 130022, China)
  • Online:2021-05-25 Published:2021-06-02

摘要: 利用DEAE-Cellulose离子交换色谱柱对豆渣可溶性多糖进行分离纯化,获得了2 种电荷均一的水溶性大豆多糖(soluble soybean polysaccharides,SSPS)SSPS-A1和SSPS-A2。进一步对组分SSPS-A1进行凝胶过滤色谱,获得电荷和分子质量均一的多糖组分SSPS-A1-c。对其进行分子质量、单糖组成、红外光谱和核磁分析。结果表明:SSPS-A1-c分子质量为31.6 kDa,主要由半乳糖醛酸(galacturonic acid,GalA)、半乳糖(galactose,Gal)和阿拉伯糖(arabinose,Ara)组成,其物质的量比为GalA∶Gal∶Ara=17.3∶44.9∶19.1;还含有少量的鼠李糖、葡萄糖和木糖;红外光谱分析表明SSPS-A1-c具有O—H、C—H和C=O等酸性多糖的特征吸收峰,同时SSPS-A1-c还存在一定程度的甲酯化或乙酰化修饰;核磁共振图谱表明,SSPS-A1-c具有α-1,5-Araf、α-t-Araf以及β-t-Galp的化学位移,推测其含有阿拉伯糖半乳聚糖(AG型)果胶结构域。同时还含有α-1,4-GalpA和CH3COO-的化学位移,推测其含有部分乙酰化的半乳糖醛酸聚糖(HG型)果胶结构域,结果和单糖组成和红外分析结果一致。研究结果将为豆渣可溶酸性多糖的应用提供理论依据,对豆渣的综合开发利用具有重要意义。

关键词: 豆渣;可溶酸性糖;分离纯化;结构解析

Abstract: The crude polysaccharide extracted from soybean dregs was purified and fractionated into two soluble acid polysaccharide fractions with uniform charge, SSPS-A1 and SSPS-A2. SSPS-A1 was further subjected to gel filtration chromatography, yielding a homogenous polysaccharide with uniform charge, SSPS-A1-c. Its molecular mass and monosaccharide composition were measured, and its structure was analyzed by infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy. The results showed that SSPS-A1-c had a molecular mass of 31.6 kDa, mainly composed of galacturonic acid (GalA), galactose (Gal) and arabinose (Ara) with a molar ratio of 17.3:44.9:19.1 together with lesser amounts of rhamnose (Rha), glucose (Glc) and xylose (Xyl). IR spectroscopy analysis showed that SSPS-A1-c had the characteristic absorption peaks of acidic polysaccharides such as O–H, C–H and C=O and it may be methylated or acetylated to a certain extent. NMR results showed that SSPS-A1-c had chemical shifts of α-1,5-Araf, α-t-Araf and β-t-Galp, which was speculated to contain arabinogalactan (AG)-type pectin domain. Besides, it also exhibited chemical shift of α-1,4-GalpA, which was inferred to contain the homogalacturonan (HG)-type pectin domain. The NMR results were consistent with the monosaccharide composition and infrared analysis results. The research results will provide a theoretical basis for the application of soluble acidic polysaccharides from soybean dregs, and will be of great significance to the comprehensive development and utilization of soybean dregs.

Key words: soybean okara; soluble acid sugar; separation and purification; structure analysis

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