产Reuterin罗伊氏乳杆菌的定向筛选及其生物学特性

doi:10.7506/spkx1002-6630-20201101-003

食品科学 ›› 2021, Vol. 42 ›› Issue (14): 32-38.doi: 10.7506/spkx1002-6630-20201101-003

产Reuterin罗伊氏乳杆菌的定向筛选及其生物学特性

李一娟，邵开生，张娜，孟迎平，陶雪莹，周颖钰，魏华，张志鸿

（1.南昌大学食品科学与技术国家重点实验室，江西南昌 330047；2.南昌大学中德联合研究院，江西南昌 330047）

发布日期:2021-07-27
基金资助:
国家自然科学基金面上项目（31770133）

Directional Screening for Reuterin-Producing Lactobacillus reuteri and Its Biological Characteristics

LI Yijuan, SHAO Kaisheng, ZHANG Na, MENG Yingping, TAO Xueying, ZHOU Yingyu, WEI Hua, ZHANG Zhihong

(1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. Sino German Joint Research Institute, Nanchang Univerisity, Nanchang 330047, China)

Published:2021-07-27

摘要/Abstract

摘要： 依据菌株宿主适应性特点，从健康家禽新鲜粪便中定向筛选具有开发潜力的罗伊氏乳杆菌。经16S rDNA序列分析和pduC基因筛查，获得3 株产抗菌物质Reuterin的罗伊氏乳杆菌，对其耐受模拟胃肠道环境、抗生素敏感性、甘油发酵上清液抑菌活性及肠上皮细胞黏附能力等生物学特性进行系统评价。结果表明，罗伊氏乳杆菌WLRE01、WLRE03、WLRE04均具有较强耐受极端酸（pH 2.5和pH 3.5）、胆盐（1.5 g/L）及模拟胃肠液的能力；经1 mmol/L过氧化氢处理6 h，WLRE03、WLRE04的活菌数能基本维持初始浓度；甘油发酵上清液对金黄色葡萄球菌具有极强的抑制作用，抑菌圈最高达（28.1±1.2）mm；3 株菌对HT-29细胞均具有良好的黏附特性，其中WLRE01黏附能力最强。综上所述，健康家禽肠道源的3 株产Reuterin的罗伊氏乳杆菌可作为益生菌候选菌株进行深入研究。

关键词: 罗伊氏乳杆菌；定向筛选；Reuterin；耐受性；抑菌；黏附

Abstract: Fresh feces from healthy poultry were collected for directional screening for candidate probiotic strains of Lactobacillus reuteri according to their host adaptation characteristics. Through 16S rDNA sequence analysis and pduC gene cluster screening, three reuterin-producing strains of L. reuteri were obtained. Their biological characteristics such as simulated gastrointestinal environment tolerance, antibiotic sensitivity, antibacterial activity of reuterin produced from glycerol by these stains and adhesion capacity to intestinal epithelial cells were evaluated. The results showed that L. reuteri WLRE01, WLRE03, and WLRE04 had strong acid (pH 2.5 and 3.5), bile salt (1.5 g/L) and simulated gastrointestinal juice resistance. After treatment with 1 mmol/L hydrogen peroxide for 6 h, the viable counts of L. reuteri WLRE03 and WLRE04 were almost kept unchanged. The produced reuterin had strong inhibitory effect on Staphylococcus aureus, with a diameter of inhibitory zone of (28.1 ± 1.2) mm. The three strains showed good adhesion capacity to HT-29 cells, and among them WLRE01 had the strongest adhesion capacity. In summary, all three strains from healthy poultry feces deserve further research as candidate probiotic strains.

Key words: Lactobacillus reuteri; directional screening; reuterin; tolerance; antibacterial activity; adhesion

中图分类号:

TS201.3

李一娟，邵开生，张娜，孟迎平，陶雪莹，周颖钰，魏华，张志鸿. 产Reuterin罗伊氏乳杆菌的定向筛选及其生物学特性[J]. 食品科学, 2021, 42(14): 32-38.

LI Yijuan, SHAO Kaisheng, ZHANG Na, MENG Yingping, TAO Xueying, ZHOU Yingyu, WEI Hua, ZHANG Zhihong. Directional Screening for Reuterin-Producing Lactobacillus reuteri and Its Biological Characteristics[J]. FOOD SCIENCE, 2021, 42(14): 32-38.

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产Reuterin罗伊氏乳杆菌的定向筛选及其生物学特性

Directional Screening for Reuterin-Producing Lactobacillus reuteri and Its Biological Characteristics

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