食品科学 ›› 2021, Vol. 42 ›› Issue (18): 157-163.doi: 10.7506/spkx1002-6630-20200707-092

• 生物工程 • 上一篇    下一篇

1 株高产几丁质脱乙酰酶红球菌的基因组测序及其应用潜力分析

肖宇,刘洋,刘建军,卢海强,桑亚新,孙纪录   

  1. (1.河北农业大学食品科技学院,河北 保定 071000;2.山东省食品发酵工业研究设计院,山东 济南 250013)
  • 发布日期:2021-09-29
  • 基金资助:
    河北省重点研发计划项目(19273201D);河北省现代农业产业技术体系淡水养殖创新团队建设项目(HBCT2018180206)

Genomic Analysis of a Rhodococcus Strain with High Chitin Deacetylase Activity and Its Application Potential

XIAO Yu, LIU Yang, LIU Jianjun, LU Haiqiang, SANG Yaxin, SUN Jilu   

  1. (1. College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China;2. Shandong Food Ferment Industry Research & Design Institute, Jinan 250013, China)
  • Published:2021-09-29

摘要: 为高效利用虾蟹壳资源,推动壳聚糖的几丁质脱乙酰酶法生产,采用Illumina测序技术,对1 株高产几丁质脱乙酰酶的红球菌菌株11-3进行基因组测序,并进行系统的生物信息学分析,主要包括基因本体论(Gene Ontology,GO)、直系同源群集(Cluster of Orthologous Group,COG)、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)功能注释,碳水化合物活性酶注释以及几丁质降解相关酶基因的生物信息学分析。研究发现,红球菌11-3的基因组为6 089 866 bp,共5 904 个编码基因,GC含量为70.514%。经碳水化合物活性酶注释共识别了165 个基因,包括59 个碳水化合物酯酶基因,42 个糖基转移酶基因,36 个糖苷水解酶基因和28 个辅助氧化还原酶基因,其中,鉴定出1 个几丁质脱乙酰酶基因(gene4907),4 个几丁质酶(EC 3.2.1.14)基因(gene1286、gene1287、gene3810、gene4754)和2 个壳聚糖酶(EC 3.2.1.132)基因(gene4921、gene5362)。gene4907与已报道的几丁质脱乙酰酶基因的序列一致性为26.60%~32.43%,为一种新的几丁质脱乙酰酶。因此,红球菌11-3菌株在几丁质资源的开发领域具有极大潜力。

关键词: 几丁质脱乙酰酶;红球菌;几丁质;基因组

Abstract: In order to efficiently utilize shrimp and crab shells for the production of chitosan with chitin deacetylase, genomic sequencing of a Rhodococcus strain with high productivity of chitin deacetylase, designed 11-3 was conducted using Illumina high-throughput sequencing in this study. Subsequently, bioinformatics analysis was performed, including Gene Ontology (GO), Cluster of Orthologous Group (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG) function annotation of genes, carbohydrate-active enzyme annotation, and bioinformatics analysis of chitin degradation enzyme genes. It was found that the genome of Rhodococcus 11-3 was 6 089 866 bp in length including a total of 5 904 coding genes with a GC content of 70.514%. A total of 165 genes were identified by carbohydrate-active enzyme annotation, including 59 carbohydrate esterase genes, 42 glycosyltransferase genes, 36 glycoside hydrolase genes and 28 auxiliary oxidoreductase genes. Among these, one chitin deacetylase gene (gene 4907), four chitinase (EC 3.2.1.14) genes (genes 1286, 1287, 3810, and 4754) and two chitinase (EC 3.2.1.132) genes (genes 4921 and 5362) were identified. The sequence similarity between gene 4907 and the reported chitin deacetylase genes was 26.60%–32.43%, suggesting that gene 4907 was a new chitin deacetylase. Therefore, Rhodococcus 11-3 has great potential in the utilization of chitin resources.

Key words: chitin deacetylase; Rhodococcus; chitin; genome

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